Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Respiratory disease and virus shedding in rhesus macaques inoculated with SARS-CoV-2

View ORCID ProfileVincent J. Munster, Friederike Feldmann, Brandi N. Williamson, Neeltje van Doremalen, Lizzette Pérez-Pérez, Jonathan Schulz, Kimberly Meade-White, Atsushi Okumura, Julie Callison, Beniah Brumbaugh, Victoria A. Avanzato, Rebecca Rosenke, Patrick W. Hanley, Greg Saturday, Dana Scott, Elizabeth R. Fischer, Emmie de Wit
doi: https://doi.org/10.1101/2020.03.21.001628
Vincent J. Munster
1Laboratory of Virology, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Vincent J. Munster
Friederike Feldmann
2Rocky Mountain Veterinary Branch, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Brandi N. Williamson
1Laboratory of Virology, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Neeltje van Doremalen
1Laboratory of Virology, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lizzette Pérez-Pérez
1Laboratory of Virology, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jonathan Schulz
1Laboratory of Virology, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kimberly Meade-White
1Laboratory of Virology, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Atsushi Okumura
1Laboratory of Virology, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julie Callison
1Laboratory of Virology, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Beniah Brumbaugh
3Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Victoria A. Avanzato
1Laboratory of Virology, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rebecca Rosenke
2Rocky Mountain Veterinary Branch, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Patrick W. Hanley
2Rocky Mountain Veterinary Branch, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Greg Saturday
2Rocky Mountain Veterinary Branch, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dana Scott
2Rocky Mountain Veterinary Branch, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elizabeth R. Fischer
3Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Emmie de Wit
1Laboratory of Virology, National Institutes of Health, Hamilton, MT, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: Emmie.dewit@nih.gov
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

An outbreak of a novel coronavirus, now named SARS-CoV-2, causing respiratory disease and a ∼2% case fatality rate started in Wuhan, China in December 2019. Following unprecedented rapid global spread, the World Health Organization declared COVID-19 a pandemic on March 11, 2020. Although data on disease in humans are emerging at a steady pace, certain aspects of the pathogenesis of SARS-CoV-2 can only be studied in detail in animal models, where repeated sampling and tissue collection is possible. Here, we show that SARS-CoV-2 causes respiratory disease in infected rhesus macaques, with disease lasting 8-16 days. Pulmonary infiltrates, a hallmark of human disease, were visible in lung radiographs of all animals. High viral loads were detected in swabs from the nose and throat of all animals as well as in bronchoalveolar lavages; in one animal we observed prolonged rectal shedding. Taken together, the rhesus macaque recapitulates moderate disease observed in the majority of human cases. The establishment of the rhesus macaque as a model of COVID-19 will increase our understanding of the pathogenesis of this disease and will aid development and testing of medical countermeasures.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. This article is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license.
Back to top
PreviousNext
Posted March 21, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Respiratory disease and virus shedding in rhesus macaques inoculated with SARS-CoV-2
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Respiratory disease and virus shedding in rhesus macaques inoculated with SARS-CoV-2
Vincent J. Munster, Friederike Feldmann, Brandi N. Williamson, Neeltje van Doremalen, Lizzette Pérez-Pérez, Jonathan Schulz, Kimberly Meade-White, Atsushi Okumura, Julie Callison, Beniah Brumbaugh, Victoria A. Avanzato, Rebecca Rosenke, Patrick W. Hanley, Greg Saturday, Dana Scott, Elizabeth R. Fischer, Emmie de Wit
bioRxiv 2020.03.21.001628; doi: https://doi.org/10.1101/2020.03.21.001628
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Respiratory disease and virus shedding in rhesus macaques inoculated with SARS-CoV-2
Vincent J. Munster, Friederike Feldmann, Brandi N. Williamson, Neeltje van Doremalen, Lizzette Pérez-Pérez, Jonathan Schulz, Kimberly Meade-White, Atsushi Okumura, Julie Callison, Beniah Brumbaugh, Victoria A. Avanzato, Rebecca Rosenke, Patrick W. Hanley, Greg Saturday, Dana Scott, Elizabeth R. Fischer, Emmie de Wit
bioRxiv 2020.03.21.001628; doi: https://doi.org/10.1101/2020.03.21.001628

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4397)
  • Biochemistry (9630)
  • Bioengineering (7123)
  • Bioinformatics (24939)
  • Biophysics (12670)
  • Cancer Biology (9995)
  • Cell Biology (14404)
  • Clinical Trials (138)
  • Developmental Biology (7989)
  • Ecology (12147)
  • Epidemiology (2067)
  • Evolutionary Biology (16025)
  • Genetics (10951)
  • Genomics (14778)
  • Immunology (9906)
  • Microbiology (23739)
  • Molecular Biology (9506)
  • Neuroscience (51051)
  • Paleontology (370)
  • Pathology (1545)
  • Pharmacology and Toxicology (2692)
  • Physiology (4038)
  • Plant Biology (8693)
  • Scientific Communication and Education (1512)
  • Synthetic Biology (2404)
  • Systems Biology (6459)
  • Zoology (1350)