Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Potent human neutralizing antibodies elicited by SARS-CoV-2 infection

Bin Ju, Qi Zhang, Xiangyang Ge, Ruoke Wang, Jiazhen Yu, Sisi Shan, Bing Zhou, Shuo Song, Xian Tang, Jinfang Yu, Jiwan Ge, Jun Lan, Jing Yuan, Haiyan Wang, Juanjuan Zhao, Shuye Zhang, Youchun Wang, Xuanling Shi, Lei Liu, Xinquan Wang, Zheng Zhang, View ORCID ProfileLinqi Zhang
doi: https://doi.org/10.1101/2020.03.21.990770
Bin Ju
1Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
2The Second Affiliated Hospital, School of Medicine, Southern University ofMN Science and Technology, Shenzhen 518055, Guangdong Province, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Qi Zhang
3Center for Global Health and Infectious Diseases, Comprehensive AIDS Research Center, and Beijing Advanced Innovation Center for Structural Biology, School of Medicine, Tsinghua University, Beijing 100084, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiangyang Ge
1Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ruoke Wang
3Center for Global Health and Infectious Diseases, Comprehensive AIDS Research Center, and Beijing Advanced Innovation Center for Structural Biology, School of Medicine, Tsinghua University, Beijing 100084, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jiazhen Yu
1Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sisi Shan
3Center for Global Health and Infectious Diseases, Comprehensive AIDS Research Center, and Beijing Advanced Innovation Center for Structural Biology, School of Medicine, Tsinghua University, Beijing 100084, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bing Zhou
1Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shuo Song
1Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xian Tang
1Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jinfang Yu
4The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center for Biological Structure, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University, 100084 Beijing, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jiwan Ge
4The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center for Biological Structure, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University, 100084 Beijing, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jun Lan
4The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center for Biological Structure, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University, 100084 Beijing, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jing Yuan
5Department for Infectious Diseases, Shenzhen Third People’s Hospital, Shenzhen, Guangdong Province 518112, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Haiyan Wang
1Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Juanjuan Zhao
1Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
2The Second Affiliated Hospital, School of Medicine, Southern University ofMN Science and Technology, Shenzhen 518055, Guangdong Province, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shuye Zhang
6Shanghai Public Health Clinical Center and Institute of Biomedical Sciences, Fudan University, Shanghai 201508, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Youchun Wang
7Division of HIV/AIDS and Sex-Transmitted Virus Vaccines, National Institutes for Food and Drug Control, Beijing, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xuanling Shi
3Center for Global Health and Infectious Diseases, Comprehensive AIDS Research Center, and Beijing Advanced Innovation Center for Structural Biology, School of Medicine, Tsinghua University, Beijing 100084, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lei Liu
1Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
2The Second Affiliated Hospital, School of Medicine, Southern University ofMN Science and Technology, Shenzhen 518055, Guangdong Province, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xinquan Wang
4The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center for Biological Structure, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University, 100084 Beijing, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zheng Zhang
1Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
2The Second Affiliated Hospital, School of Medicine, Southern University ofMN Science and Technology, Shenzhen 518055, Guangdong Province, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: zhangzheng1975@aliyun.com zhanglinqi@tsinghua.edu.cn
Linqi Zhang
3Center for Global Health and Infectious Diseases, Comprehensive AIDS Research Center, and Beijing Advanced Innovation Center for Structural Biology, School of Medicine, Tsinghua University, Beijing 100084, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Linqi Zhang
  • For correspondence: zhangzheng1975@aliyun.com zhanglinqi@tsinghua.edu.cn
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

The pandemic caused by emerging coronavirus SARS-CoV-2 presents a serious global public health emergency in urgent need of prophylactic and therapeutic interventions. SARS-CoV-2 cellular entry depends on binding between the viral Spike protein receptor-binding domain (RBD) and the angiotensin converting enzyme 2 (ACE2) target cell receptor. Here, we report on the isolation and characterization of 206 RBD-specific monoclonal antibodies (mAbs) derived from single B cells of eight SARS-CoV-2 infected individuals. These mAbs come from diverse families of antibody heavy and light chains without apparent enrichment for particular families in the repertoire. In samples from one patient selected for further analyses, we found coexistence of germline and germline divergent clones. Both clone types demonstrated impressive binding and neutralizing activity against pseudovirus and live SARS-CoV-2. However, the antibody neutralizing potency is determined by competition with ACE2 receptor for RBD binding. Surprisingly, none of the SARS-CoV-2 antibodies nor the infected plasma cross-reacted with RBDs from either SARS-CoV or MERS-CoV although substantial plasma cross-reactivity to the trimeric Spike proteins from SARS-CoV and MERS-CoV was found. These results suggest that antibody response to RBDs is viral species-specific while that cross-recognition target regions outside the RBD. The specificity and neutralizing characteristics of this plasma cross-reactivity requires further investigation. Nevertheless, the diverse and potent neutralizing antibodies identified here are promising candidates for prophylactic and therapeutic SARS-CoV-2 interventions.

Footnotes

  • Figure 4 revised.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted March 26, 2020.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Potent human neutralizing antibodies elicited by SARS-CoV-2 infection
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Potent human neutralizing antibodies elicited by SARS-CoV-2 infection
Bin Ju, Qi Zhang, Xiangyang Ge, Ruoke Wang, Jiazhen Yu, Sisi Shan, Bing Zhou, Shuo Song, Xian Tang, Jinfang Yu, Jiwan Ge, Jun Lan, Jing Yuan, Haiyan Wang, Juanjuan Zhao, Shuye Zhang, Youchun Wang, Xuanling Shi, Lei Liu, Xinquan Wang, Zheng Zhang, Linqi Zhang
bioRxiv 2020.03.21.990770; doi: https://doi.org/10.1101/2020.03.21.990770
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Potent human neutralizing antibodies elicited by SARS-CoV-2 infection
Bin Ju, Qi Zhang, Xiangyang Ge, Ruoke Wang, Jiazhen Yu, Sisi Shan, Bing Zhou, Shuo Song, Xian Tang, Jinfang Yu, Jiwan Ge, Jun Lan, Jing Yuan, Haiyan Wang, Juanjuan Zhao, Shuye Zhang, Youchun Wang, Xuanling Shi, Lei Liu, Xinquan Wang, Zheng Zhang, Linqi Zhang
bioRxiv 2020.03.21.990770; doi: https://doi.org/10.1101/2020.03.21.990770

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Immunology
Subject Areas
All Articles
  • Animal Behavior and Cognition (2639)
  • Biochemistry (5231)
  • Bioengineering (3653)
  • Bioinformatics (15748)
  • Biophysics (7226)
  • Cancer Biology (5604)
  • Cell Biology (8060)
  • Clinical Trials (138)
  • Developmental Biology (4748)
  • Ecology (7477)
  • Epidemiology (2059)
  • Evolutionary Biology (10535)
  • Genetics (7708)
  • Genomics (10093)
  • Immunology (5168)
  • Microbiology (13844)
  • Molecular Biology (5361)
  • Neuroscience (30629)
  • Paleontology (213)
  • Pathology (873)
  • Pharmacology and Toxicology (1520)
  • Physiology (2236)
  • Plant Biology (4992)
  • Scientific Communication and Education (1039)
  • Synthetic Biology (1382)
  • Systems Biology (4135)
  • Zoology (808)