Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Genetically Encoded, Multivalent Liquid Glycan Array (LiGA)

Mirat Sojitra, Susmita Sarkar, Jasmine Maghera, Emily Rodrigues, Eric Carpenter, Shaurya Seth, Daniel Ferrer Vinals, Nicholas Bennett, Revathi Reddy, Amira Khalil, View ORCID ProfileXiaochao Xue, Michael Bell, View ORCID ProfileRuixiang Blake Zheng, Chang-Chun Ling, View ORCID ProfileTodd L. Lowary, James C. Paulson, Matthew S. Macauley, View ORCID ProfileRatmir Derda
doi: https://doi.org/10.1101/2020.03.24.997536
Mirat Sojitra
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Susmita Sarkar
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jasmine Maghera
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Emily Rodrigues
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eric Carpenter
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shaurya Seth
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Daniel Ferrer Vinals
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nicholas Bennett
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Revathi Reddy
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Amira Khalil
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiaochao Xue
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Xiaochao Xue
Michael Bell
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ruixiang Blake Zheng
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ruixiang Blake Zheng
Chang-Chun Ling
2Department of Chemistry, University of Calgary, Calgary, AB T2N 1N4, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Todd L. Lowary
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
5Institute of Biological Chemistry, Academia Sinica, Academia Road, Section 2, #128, Nangang, Taipei 11529, Taiwan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Todd L. Lowary
James C. Paulson
3Department of Molecular Medicine, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Matthew S. Macauley
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
4Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, AB T6G 2G2, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ratmir Derda
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ratmir Derda
  • For correspondence: ratmir@ualberta.ca
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Data/Code
  • Preview PDF
Loading

Abstract

The Central Dogma of Biology does not allow for the study of glycans using DNA sequencing. We report a “Liquid Glycan Array” (LiGA) platform comprising a library of DNA ‘barcoded’ M13 virions that display 30-1500 copies of glycans per phage. A LiGA is synthesized by acylation of phage pVIII protein with a dibenzocyclooctyne, followed by ligation of azido-modified glycans. Pulldown of the LiGA with lectins followed by deep sequencing of the barcodes in the bound phage decodes the optimal structure and density of the recognized glycans. The LiGA is target agnostic and can measure the glycan-binding profile of lectins such as CD22 on cells in vitro and immune cells in a live mouse. From a mixture of multivalent glycan probes, LiGAs identifies the glycoconjugates with optimal avidity necessary for binding to lectins on living cells in vitro and in vivo; measurements that cannot be performed with canonical glass slide-based glycan arrays.

Footnotes

  • Dedication: The paper is dedicated to Laura L. Kiessling on the occasion of her 60th birthday.

  • http://ligacloud.ca/

  • https://github.com/derdalab/liga

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted March 25, 2020.
Download PDF

Supplementary Material

Data/Code
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Genetically Encoded, Multivalent Liquid Glycan Array (LiGA)
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Genetically Encoded, Multivalent Liquid Glycan Array (LiGA)
Mirat Sojitra, Susmita Sarkar, Jasmine Maghera, Emily Rodrigues, Eric Carpenter, Shaurya Seth, Daniel Ferrer Vinals, Nicholas Bennett, Revathi Reddy, Amira Khalil, Xiaochao Xue, Michael Bell, Ruixiang Blake Zheng, Chang-Chun Ling, Todd L. Lowary, James C. Paulson, Matthew S. Macauley, Ratmir Derda
bioRxiv 2020.03.24.997536; doi: https://doi.org/10.1101/2020.03.24.997536
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Genetically Encoded, Multivalent Liquid Glycan Array (LiGA)
Mirat Sojitra, Susmita Sarkar, Jasmine Maghera, Emily Rodrigues, Eric Carpenter, Shaurya Seth, Daniel Ferrer Vinals, Nicholas Bennett, Revathi Reddy, Amira Khalil, Xiaochao Xue, Michael Bell, Ruixiang Blake Zheng, Chang-Chun Ling, Todd L. Lowary, James C. Paulson, Matthew S. Macauley, Ratmir Derda
bioRxiv 2020.03.24.997536; doi: https://doi.org/10.1101/2020.03.24.997536

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biochemistry
Subject Areas
All Articles
  • Animal Behavior and Cognition (4095)
  • Biochemistry (8786)
  • Bioengineering (6493)
  • Bioinformatics (23386)
  • Biophysics (11766)
  • Cancer Biology (9167)
  • Cell Biology (13290)
  • Clinical Trials (138)
  • Developmental Biology (7422)
  • Ecology (11386)
  • Epidemiology (2066)
  • Evolutionary Biology (15119)
  • Genetics (10413)
  • Genomics (14024)
  • Immunology (9145)
  • Microbiology (22108)
  • Molecular Biology (8793)
  • Neuroscience (47445)
  • Paleontology (350)
  • Pathology (1423)
  • Pharmacology and Toxicology (2483)
  • Physiology (3711)
  • Plant Biology (8063)
  • Scientific Communication and Education (1433)
  • Synthetic Biology (2215)
  • Systems Biology (6021)
  • Zoology (1251)