Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

The cytosolic Arabidopsis thaliana cysteine desulfurase ABA3 delivers sulfur to the sulfurtransferase STR18

Benjamin Selles, Anna Moseler, Damien Caubrière, Sheng-Kai Sun, Morgane Ziesel, Tiphaine Dhalleine, Mathilde Hériché, Markus Wirtz, View ORCID ProfileNicolas Rouhier, View ORCID ProfileJérémy Couturier
doi: https://doi.org/10.1101/2020.03.25.008375
Benjamin Selles
1Université de Lorraine, INRAE, IAM, F-54000 Nancy, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anna Moseler
1Université de Lorraine, INRAE, IAM, F-54000 Nancy, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Damien Caubrière
1Université de Lorraine, INRAE, IAM, F-54000 Nancy, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sheng-Kai Sun
2Centre for Organismal Studies (COS), University of Heidelberg, 69120, Heidelberg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Morgane Ziesel
1Université de Lorraine, INRAE, IAM, F-54000 Nancy, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tiphaine Dhalleine
1Université de Lorraine, INRAE, IAM, F-54000 Nancy, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mathilde Hériché
1Université de Lorraine, INRAE, IAM, F-54000 Nancy, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Markus Wirtz
2Centre for Organismal Studies (COS), University of Heidelberg, 69120, Heidelberg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nicolas Rouhier
1Université de Lorraine, INRAE, IAM, F-54000 Nancy, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Nicolas Rouhier
Jérémy Couturier
1Université de Lorraine, INRAE, IAM, F-54000 Nancy, France
3Institut Universitaire de France, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jérémy Couturier
  • For correspondence: jeremy.couturier@univ-lorraine.fr
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

The biosynthesis of many sulfur-containing molecules depends on cysteine as a sulfur source. Cysteine desulfurase (CD) and rhodanese (Rhd) domain-containing protein families participate in the trafficking of sulfur for various metabolic pathways in bacteria and human, but their connection is not yet described in plants. The existence of natural chimeric proteins, however, containing both CD and Rhd domains in specific bacterial genera suggests a general interaction between both proteins. We report here the biochemical relationships between two cytosolic proteins from Arabidopsis thaliana, a Rhd domain containing protein, the sulfurtransferase 18 (STR18), and a CD isoform referred to as ABA3, and compare these biochemical features to those of a natural CD-Rhd fusion protein from the bacterium Pseudorhodoferax sp.. We observed that the bacterial enzyme is bifunctional exhibiting both CD and STR activities using L-cysteine and thiosulfate as sulfur donors but preferentially uses L-cysteine to catalyze trans-persulfidation reactions. In vitro activity assays and mass spectrometry analyses revealed that STR18 stimulates the CD activity of ABA3 by reducing the intermediate persulfide on its catalytic cysteine thereby accelerating the overall transfer reaction. Both proteins interact in planta and form an efficient sulfur relay system whereby STR18 catalyzes trans-persulfidation reactions from ABA3 to the model acceptor protein roGFP2. In conclusion, the ABA3-STR18 couple likely represents an uncharacterized pathway of sulfur trafficking in the cytosol of plant cells, independent of ABA3 function in molybdenum cofactor maturation.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted January 10, 2022.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
The cytosolic Arabidopsis thaliana cysteine desulfurase ABA3 delivers sulfur to the sulfurtransferase STR18
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
The cytosolic Arabidopsis thaliana cysteine desulfurase ABA3 delivers sulfur to the sulfurtransferase STR18
Benjamin Selles, Anna Moseler, Damien Caubrière, Sheng-Kai Sun, Morgane Ziesel, Tiphaine Dhalleine, Mathilde Hériché, Markus Wirtz, Nicolas Rouhier, Jérémy Couturier
bioRxiv 2020.03.25.008375; doi: https://doi.org/10.1101/2020.03.25.008375
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
The cytosolic Arabidopsis thaliana cysteine desulfurase ABA3 delivers sulfur to the sulfurtransferase STR18
Benjamin Selles, Anna Moseler, Damien Caubrière, Sheng-Kai Sun, Morgane Ziesel, Tiphaine Dhalleine, Mathilde Hériché, Markus Wirtz, Nicolas Rouhier, Jérémy Couturier
bioRxiv 2020.03.25.008375; doi: https://doi.org/10.1101/2020.03.25.008375

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Plant Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4114)
  • Biochemistry (8816)
  • Bioengineering (6519)
  • Bioinformatics (23465)
  • Biophysics (11792)
  • Cancer Biology (9211)
  • Cell Biology (13325)
  • Clinical Trials (138)
  • Developmental Biology (7439)
  • Ecology (11413)
  • Epidemiology (2066)
  • Evolutionary Biology (15155)
  • Genetics (10439)
  • Genomics (14045)
  • Immunology (9173)
  • Microbiology (22159)
  • Molecular Biology (8813)
  • Neuroscience (47577)
  • Paleontology (350)
  • Pathology (1429)
  • Pharmacology and Toxicology (2492)
  • Physiology (3731)
  • Plant Biology (8082)
  • Scientific Communication and Education (1437)
  • Synthetic Biology (2221)
  • Systems Biology (6039)
  • Zoology (1253)