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Site-specific analysis of the SARS-CoV-2 glycan shield

View ORCID ProfileYasunori Watanabe, View ORCID ProfileJoel D. Allen, Daniel Wrapp, Jason S. McLellan, View ORCID ProfileMax Crispin
doi: https://doi.org/10.1101/2020.03.26.010322
Yasunori Watanabe
1School of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK
2Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK
3Division of Structural Biology, University of Oxford, Wellcome Centre for Human Genetics, Oxford, OX3 7BN, UK
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Joel D. Allen
1School of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK
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Daniel Wrapp
4Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA
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Jason S. McLellan
4Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA
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Max Crispin
1School of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK
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  • ORCID record for Max Crispin
  • For correspondence: max.crispin@soton.ac.uk
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Abstract

The emergence of the betacoronavirus, SARS-CoV-2 that causes COVID-19, represents a significant threat to global human health. Vaccine development is focused on the principal target of the humoral immune response, the spike (S) glycoprotein, that mediates cell entry and membrane fusion. SARS-CoV-2 S gene encodes 22 N-linked glycan sequons per protomer, which likely play a role in immune evasion and occluding immunogenic protein epitopes. Here, using a site-specific mass spectrometric approach, we reveal the glycan structures on a recombinant SARS-CoV-2 S immunogen. This analysis enables mapping of the glycan-processing states across the trimeric viral spike. We show how SARS-CoV-2 S glycans differ from typical host glycan processing, which may have implications in viral pathobiology and vaccine design.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted March 28, 2020.
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Site-specific analysis of the SARS-CoV-2 glycan shield
Yasunori Watanabe, Joel D. Allen, Daniel Wrapp, Jason S. McLellan, Max Crispin
bioRxiv 2020.03.26.010322; doi: https://doi.org/10.1101/2020.03.26.010322
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Site-specific analysis of the SARS-CoV-2 glycan shield
Yasunori Watanabe, Joel D. Allen, Daniel Wrapp, Jason S. McLellan, Max Crispin
bioRxiv 2020.03.26.010322; doi: https://doi.org/10.1101/2020.03.26.010322

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