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Morphological peculiarities of DNA-protein complexes in dormant Escherichia coli cells, subjected to prolonged starvation Condensation of DNA in dormant cells of Escherichia coli

Natalia Loiko, Yana Danilova, Andrey Moiseenko, Vladislav Kovalenko, Ksenia Tereshkina, Galina I. El-Registan, Olga S. Sokolova, View ORCID ProfileYurii F. Krupyanskii
doi: https://doi.org/10.1101/2020.03.27.011494
Natalia Loiko
1Department of Structure of Matter, Semenov Federal Research Center of Chemical Physics, RAS, Moscow, Russia
2Department of Microbiology, Federal Research Center ‘Fundamentals of Biotechnology’ RAS, Moscow, Russia
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Yana Danilova
3Department of Biology, Lomonosov Moscow State University, Faculty of Biology, Moscow, Russia
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Andrey Moiseenko
1Department of Structure of Matter, Semenov Federal Research Center of Chemical Physics, RAS, Moscow, Russia
3Department of Biology, Lomonosov Moscow State University, Faculty of Biology, Moscow, Russia
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Vladislav Kovalenko
1Department of Structure of Matter, Semenov Federal Research Center of Chemical Physics, RAS, Moscow, Russia
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Ksenia Tereshkina
1Department of Structure of Matter, Semenov Federal Research Center of Chemical Physics, RAS, Moscow, Russia
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Galina I. El-Registan
2Department of Microbiology, Federal Research Center ‘Fundamentals of Biotechnology’ RAS, Moscow, Russia
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Olga S. Sokolova
3Department of Biology, Lomonosov Moscow State University, Faculty of Biology, Moscow, Russia
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  • For correspondence: yuriifkru@gmail.com sokolova@mail.bio.msu.ru
Yurii F. Krupyanskii
1Department of Structure of Matter, Semenov Federal Research Center of Chemical Physics, RAS, Moscow, Russia
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  • ORCID record for Yurii F. Krupyanskii
  • For correspondence: yuriifkru@gmail.com sokolova@mail.bio.msu.ru
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Abstract

One of the adaptive strategies for the constantly changing conditions of the environment utilized in bacterial cells involves the condensation of DNA in complex with the DNA-binding protein, Dps. With the use of electron microscopy and electron tomography, we observed several morphologically different types of DNA condensation in dormant Escherichia coli cells, namely: nanocrystalline, liquid crystalline, and the folded nucleosome-like. We confirmed the presence of both Dps and DNA in all of the ordered structures using EDX analysis. The comparison of EDX spectra obtained for the three different ordered structures revealed that in nanocrystalline formation the majority of Dps protein is tightly bound to nucleoid DNA. We demonstrated that the population of the dormant cell is structurally heterogeneous, which allows cells to respond flexibly to environmental changes. It increases the ability of the whole bacterial population to survive under extreme stress conditions.

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Posted March 27, 2020.
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Morphological peculiarities of DNA-protein complexes in dormant Escherichia coli cells, subjected to prolonged starvation Condensation of DNA in dormant cells of Escherichia coli
Natalia Loiko, Yana Danilova, Andrey Moiseenko, Vladislav Kovalenko, Ksenia Tereshkina, Galina I. El-Registan, Olga S. Sokolova, Yurii F. Krupyanskii
bioRxiv 2020.03.27.011494; doi: https://doi.org/10.1101/2020.03.27.011494
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Morphological peculiarities of DNA-protein complexes in dormant Escherichia coli cells, subjected to prolonged starvation Condensation of DNA in dormant cells of Escherichia coli
Natalia Loiko, Yana Danilova, Andrey Moiseenko, Vladislav Kovalenko, Ksenia Tereshkina, Galina I. El-Registan, Olga S. Sokolova, Yurii F. Krupyanskii
bioRxiv 2020.03.27.011494; doi: https://doi.org/10.1101/2020.03.27.011494

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