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Left-handed DNA-PAINT for improved superresolution imaging in the nucleus

View ORCID ProfileH.J. Geertsema, G. Aimola, V. Fabricius, J.P. Fuerste, View ORCID ProfileB.B. Kaufer, View ORCID ProfileH. Ewers
doi: https://doi.org/10.1101/2020.03.28.010553
H.J. Geertsema
1Institute for Chemistry and Biochemistry, Department for Biology, Chemistry and Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany
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G. Aimola
2Institut for Virology, Department of Veterinary Science, Freie Universität Berlin, 14163 Berlin, Germany
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V. Fabricius
1Institute for Chemistry and Biochemistry, Department for Biology, Chemistry and Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany
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J.P. Fuerste
1Institute for Chemistry and Biochemistry, Department for Biology, Chemistry and Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany
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B.B. Kaufer
2Institut for Virology, Department of Veterinary Science, Freie Universität Berlin, 14163 Berlin, Germany
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H. Ewers
1Institute for Chemistry and Biochemistry, Department for Biology, Chemistry and Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany
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  • For correspondence: helge.ewers@fu-berlin.de
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Abstract

DNA point accumulation in nanoscale topography (DNA-PAINT) advances super-resolution microscopy with superior resolution and multiplexing capabilities. However, cellular DNA may interfere with this single-molecule localization technique based on DNA-DNA hybridization. Here, we introduce left-handed DNA (L-DNA) oligomers that do not hybridize to naturally present R-DNA and demonstrate that L-DNA PAINT has the same specificity and multiplexing capability as R-DNA PAINT, but greatly improves specific visualization of nuclear target molecules.

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Posted March 29, 2020.
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Left-handed DNA-PAINT for improved superresolution imaging in the nucleus
H.J. Geertsema, G. Aimola, V. Fabricius, J.P. Fuerste, B.B. Kaufer, H. Ewers
bioRxiv 2020.03.28.010553; doi: https://doi.org/10.1101/2020.03.28.010553
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Left-handed DNA-PAINT for improved superresolution imaging in the nucleus
H.J. Geertsema, G. Aimola, V. Fabricius, J.P. Fuerste, B.B. Kaufer, H. Ewers
bioRxiv 2020.03.28.010553; doi: https://doi.org/10.1101/2020.03.28.010553

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