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Genetically controlled membrane synthesis in liposomes

Duco Blanken, David Foschepoth, Adriana Calaça Serrão, Christophe Danelon
doi: https://doi.org/10.1101/2020.03.29.013300
Duco Blanken
Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, van der Maasweg 9, 2629 HZ, Delft, The Netherlands
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David Foschepoth
Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, van der Maasweg 9, 2629 HZ, Delft, The Netherlands
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Adriana Calaça Serrão
Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, van der Maasweg 9, 2629 HZ, Delft, The Netherlands
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Christophe Danelon
Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, van der Maasweg 9, 2629 HZ, Delft, The Netherlands
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  • For correspondence: c.j.a.danelon@tudelft.nl
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ABSTRACT

Lipid membrane, nucleic acids, proteins, and metabolism are essential ingredients for life. Synthetic cellular systems emulating the fundamental properties of living cells must therefore be built upon these functional elements. In this work, phospholipid-producing enzymes encoded in a synthetic minigenome are cell-free expressed within liposome compartments. The de novo synthesized metabolic pathway converts precursors into a variety of lipids, including the constituents of the parental liposome. Balanced production of phosphatidylethanolamine and phosphatidylglycerol is realized, owing to transcriptional regulation of specific genes activity combined with a metabolic feedback mechanism. Fluorescence-based methods are developed to image the synthesis and membrane incorporation of phosphatidylserine at the single liposome level. Our results provide experimental evidence for DNA-programmed membrane synthesis in a minimal cell model. Strategies are discussed to alleviate current limitations toward more effective liposome growth and self-reproduction.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted March 30, 2020.
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Genetically controlled membrane synthesis in liposomes
Duco Blanken, David Foschepoth, Adriana Calaça Serrão, Christophe Danelon
bioRxiv 2020.03.29.013300; doi: https://doi.org/10.1101/2020.03.29.013300
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Genetically controlled membrane synthesis in liposomes
Duco Blanken, David Foschepoth, Adriana Calaça Serrão, Christophe Danelon
bioRxiv 2020.03.29.013300; doi: https://doi.org/10.1101/2020.03.29.013300

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