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Combined prophylactic and therapeutic use maximizes hydroxychloroquine anti-SARS-CoV-2 effects in vitro

View ORCID ProfileNicola Clementi, Elena Criscuolo, Roberta Antonia Diotti, Roberto Ferrarese, Matteo Castelli, Roberto Burioni, Massimo Clementi, Nicasio Mancini
doi: https://doi.org/10.1101/2020.03.29.014407
Nicola Clementi
1Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, Milano, Italia
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  • ORCID record for Nicola Clementi
Elena Criscuolo
1Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, Milano, Italia
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Roberta Antonia Diotti
1Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, Milano, Italia
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Roberto Ferrarese
1Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, Milano, Italia
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Matteo Castelli
1Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, Milano, Italia
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Roberto Burioni
1Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, Milano, Italia
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Massimo Clementi
1Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, Milano, Italia
2IRCCS Ospedale San Raffaele, Milano, Italia
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Nicasio Mancini
1Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, Milano, Italia
2IRCCS Ospedale San Raffaele, Milano, Italia
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  • For correspondence: mancini.nicasio@hsr.it
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Abstract

While the SARS-CoV-2 pandemic is hardly hitting the world, it is of extreme importance that significant in vitro observations guide the quick set up of clinical trials. In this study, we evidence that the anti-SARS-CoV2 activity of a clinically achievable hydroxychloroquine concentration is maximized only when administered before and after the infection of Vero E6 cells. This strongly suggests that only a combined prophylactic and therapeutic use of hydroxychloroquine may be effective in limiting viral replication in patients.

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Posted March 31, 2020.
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Combined prophylactic and therapeutic use maximizes hydroxychloroquine anti-SARS-CoV-2 effects in vitro
Nicola Clementi, Elena Criscuolo, Roberta Antonia Diotti, Roberto Ferrarese, Matteo Castelli, Roberto Burioni, Massimo Clementi, Nicasio Mancini
bioRxiv 2020.03.29.014407; doi: https://doi.org/10.1101/2020.03.29.014407
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Combined prophylactic and therapeutic use maximizes hydroxychloroquine anti-SARS-CoV-2 effects in vitro
Nicola Clementi, Elena Criscuolo, Roberta Antonia Diotti, Roberto Ferrarese, Matteo Castelli, Roberto Burioni, Massimo Clementi, Nicasio Mancini
bioRxiv 2020.03.29.014407; doi: https://doi.org/10.1101/2020.03.29.014407

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