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Directed manipulation of membrane proteins by fluorescent magnetic nanoparticles

View ORCID ProfileJia Hui Li, Paula Santos-Otte, Braedyn Au, Jakob Rentsch, Stephan Block, View ORCID ProfileHelge Ewers
doi: https://doi.org/10.1101/2020.03.30.016477
Jia Hui Li
1Institut für Biochemie, Freie Universität Berlin, Thielallee 63, 14195 Berlin
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Paula Santos-Otte
1Institut für Biochemie, Freie Universität Berlin, Thielallee 63, 14195 Berlin
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Braedyn Au
1Institut für Biochemie, Freie Universität Berlin, Thielallee 63, 14195 Berlin
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Jakob Rentsch
1Institut für Biochemie, Freie Universität Berlin, Thielallee 63, 14195 Berlin
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Stephan Block
2Institut für Chemie, Freie Universität Berlin, Takustr. 3, 14195 Berlin, Germany
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Helge Ewers
1Institut für Biochemie, Freie Universität Berlin, Thielallee 63, 14195 Berlin
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  • ORCID record for Helge Ewers
  • For correspondence: helge.ewers@fu-berlin.de
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Abstract

The plasma membrane is the interface through which cells interact with their environment. Membrane proteins are embedded in the lipid bilayer of the plasma membrane and their function in this context is often linked to their specific location and dynamics within the membrane. However, few methods are available for nanoscale manipulation of membrane protein location at the single molecule level. Here, we report the use of fluorescent magnetic nanoparticles (FMNPs) to track membrane molecules and to manipulate their movement. FMNPs allow single-particle tracking (SPT) at 10 nm spatial and 5 ms temporal resolution, and using a magnetic needle, we pull membrane components laterally through the membrane with femtonewton-range forces. In this way, we successfully dragged lipid-anchored and transmembrane proteins over the surface of living cells. Doing so, we detected submembrane barriers and in combination with super-resolution microscopy could localize these barriers to the actin cytoskeleton. We present here a versatile approach to probe membrane processes in live cells via the magnetic control of membrane protein motion.

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Posted April 01, 2020.
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Directed manipulation of membrane proteins by fluorescent magnetic nanoparticles
Jia Hui Li, Paula Santos-Otte, Braedyn Au, Jakob Rentsch, Stephan Block, Helge Ewers
bioRxiv 2020.03.30.016477; doi: https://doi.org/10.1101/2020.03.30.016477
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Directed manipulation of membrane proteins by fluorescent magnetic nanoparticles
Jia Hui Li, Paula Santos-Otte, Braedyn Au, Jakob Rentsch, Stephan Block, Helge Ewers
bioRxiv 2020.03.30.016477; doi: https://doi.org/10.1101/2020.03.30.016477

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