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Evolution of cellular diversity in primary motor cortex of human, marmoset monkey, and mouse

View ORCID ProfileTrygve E. Bakken, View ORCID ProfileNikolas L. Jorstad, View ORCID ProfileQiwen Hu, View ORCID ProfileBlue B. Lake, View ORCID ProfileWei Tian, View ORCID ProfileBrian E. Kalmbach, View ORCID ProfileMegan Crow, View ORCID ProfileRebecca D. Hodge, View ORCID ProfileFenna M. Krienen, View ORCID ProfileStaci A. Sorensen, View ORCID ProfileJeroen Eggermont, View ORCID ProfileZizhen Yao, View ORCID ProfileBrian D. Aevermann, View ORCID ProfileAndrew I. Aldridge, View ORCID ProfileAnna Bartlett, View ORCID ProfileDarren Bertagnolli, View ORCID ProfileTamara Casper, View ORCID ProfileRosa G. Castanon, View ORCID ProfileKirsten Crichton, View ORCID ProfileTanya L. Daigle, View ORCID ProfileRachel Dalley, View ORCID ProfileNick Dee, View ORCID ProfileNikolai Dembrow, View ORCID ProfileDinh Diep, View ORCID ProfileSong-Lin Ding, View ORCID ProfileWeixiu Dong, View ORCID ProfileRongxin Fang, View ORCID ProfileStephan Fischer, View ORCID ProfileMelissa Goldman, View ORCID ProfileJeff Goldy, View ORCID ProfileLucas T. Graybuck, View ORCID ProfileBrian R. Herb, View ORCID ProfileXiaomeng Hou, View ORCID ProfileJayaram Kancherla, View ORCID ProfileMatthew Kroll, View ORCID ProfileKanan Lathia, View ORCID ProfileBaldur van Lew, View ORCID ProfileYang Eric Li, View ORCID ProfileChristine S. Liu, View ORCID ProfileHanqing Liu, View ORCID ProfileJacinta D. Lucero, View ORCID ProfileAnup Mahurkar, View ORCID ProfileDelissa McMillen, View ORCID ProfileJeremy A. Miller, View ORCID ProfileMarmar Moussa, View ORCID ProfileJoseph R. Nery, View ORCID ProfilePhilip R. Nicovich, View ORCID ProfileJoshua Orvis, View ORCID ProfileJulia K. Osteen, View ORCID ProfileScott Owen, View ORCID ProfileCarter R. Palmer, View ORCID ProfileThanh Pham, View ORCID ProfileNongluk Plongthongkum, View ORCID ProfileOlivier Poirion, View ORCID ProfileNora M. Reed, View ORCID ProfileChristine Rimorin, View ORCID ProfileAngeline Rivkin, View ORCID ProfileWilliam J. Romanow, View ORCID ProfileAdriana E. Sedeño-Cortés, View ORCID ProfileKimberly Siletti, View ORCID ProfileSaroja Somasundaram, View ORCID ProfileJosef Sulc, View ORCID ProfileMichael Tieu, View ORCID ProfileAmy Torkelson, View ORCID ProfileHerman Tung, View ORCID ProfileXinxin Wang, View ORCID ProfileFangming Xie, View ORCID ProfileAnna Marie Yanny, View ORCID ProfileRenee Zhang, View ORCID ProfileSeth A. Ament, View ORCID ProfileM. Margarita Behrens, View ORCID ProfileHector Corrada Bravo, View ORCID ProfileJerold Chun, View ORCID ProfileAlexander Dobin, View ORCID ProfileJesse Gillis, View ORCID ProfileRonna Hertzano, View ORCID ProfilePatrick R. Hof, View ORCID ProfileThomas Höllt, View ORCID ProfileGregory D. Horwitz, View ORCID ProfileC. Dirk Keene, View ORCID ProfilePeter V. Kharchenko, View ORCID ProfileAndrew L. Ko, View ORCID ProfileBoudewijn P. Lelieveldt, View ORCID ProfileChongyuan Luo, View ORCID ProfileEran A. Mukamel, View ORCID ProfileSebastian Preissl, View ORCID ProfileAviv Regev, View ORCID ProfileBing Ren, View ORCID ProfileRichard H. Scheuermann, View ORCID ProfileKimberly Smith, View ORCID ProfileWilliam J. Spain, View ORCID ProfileOwen R. White, View ORCID ProfileChristof Koch, View ORCID ProfileMichael Hawrylycz, View ORCID ProfileBosiljka Tasic, View ORCID ProfileEvan Z. Macosko, View ORCID ProfileSteven A. McCarroll, View ORCID ProfileJonathan T. Ting, View ORCID ProfileHongkui Zeng, View ORCID ProfileKun Zhang, View ORCID ProfileGuoping Feng, View ORCID ProfileJoseph R. Ecker, View ORCID ProfileSten Linnarsson, View ORCID ProfileEd S. Lein
doi: https://doi.org/10.1101/2020.03.31.016972
Trygve E. Bakken
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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  • For correspondence: edl@alleninstitute.org trygveb@alleninstitute.org
Nikolas L. Jorstad
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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  • ORCID record for Nikolas L. Jorstad
Qiwen Hu
2Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA
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  • ORCID record for Qiwen Hu
Blue B. Lake
3Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
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  • ORCID record for Blue B. Lake
Wei Tian
4Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
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  • ORCID record for Wei Tian
Brian E. Kalmbach
5Allen Institute for Brain Science, Seattle, WA 98109, USA; University of Washington, Department of Physiology and Biophysics, Seattle, WA 98195, USA
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  • ORCID record for Brian E. Kalmbach
Megan Crow
6Stanley Institute for Cognitive Genomics, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
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  • ORCID record for Megan Crow
Rebecca D. Hodge
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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  • ORCID record for Rebecca D. Hodge
Fenna M. Krienen
7Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
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  • ORCID record for Fenna M. Krienen
Staci A. Sorensen
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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  • ORCID record for Staci A. Sorensen
Jeroen Eggermont
8LKEB, Department of Radiology, Leiden University Medical Center, Leiden, the Netherlands
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  • ORCID record for Jeroen Eggermont
Zizhen Yao
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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  • ORCID record for Zizhen Yao
Brian D. Aevermann
9J. Craig Venter Institute, La Jolla, CA 92037, USA
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  • ORCID record for Brian D. Aevermann
Andrew I. Aldridge
4Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
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  • ORCID record for Andrew I. Aldridge
Anna Bartlett
4Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
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  • ORCID record for Anna Bartlett
Darren Bertagnolli
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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  • ORCID record for Darren Bertagnolli
Tamara Casper
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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  • ORCID record for Tamara Casper
Rosa G. Castanon
4Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
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  • ORCID record for Rosa G. Castanon
Kirsten Crichton
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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  • ORCID record for Kirsten Crichton
Tanya L. Daigle
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Rachel Dalley
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Nick Dee
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Nikolai Dembrow
10Epilepsy Center of Excellence, Department of Veteran's Affairs Medical Center, Seattle, WA 98108, USA; Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA
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Dinh Diep
3Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
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  • ORCID record for Dinh Diep
Song-Lin Ding
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Weixiu Dong
3Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
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Rongxin Fang
11Bioinformatics and Systems Biology Graduate Program, UC San Diego, La Jolla, CA 92037, USA
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  • ORCID record for Rongxin Fang
Stephan Fischer
6Stanley Institute for Cognitive Genomics, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
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  • ORCID record for Stephan Fischer
Melissa Goldman
7Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
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Jeff Goldy
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Lucas T. Graybuck
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Brian R. Herb
12Institute for Genomes Sciences, University of Maryland School of Medicine, Baltimore, MD 21201
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Xiaomeng Hou
13Center for Epigenomics, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
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Jayaram Kancherla
14Department of Computer Science, University of Maryland College Park, College Park, MD 20742, USA
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Matthew Kroll
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Kanan Lathia
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Baldur van Lew
15LKEB, Dept of Radiology, Leiden University Medical Center, Leiden, the Netherlands
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Yang Eric Li
16Ludwig Institute for Cancer Research, La Jolla, CA 92093, USA
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Christine S. Liu
17Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, USA; Biomedical Sciences Program, School of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
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  • ORCID record for Christine S. Liu
Hanqing Liu
4Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
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Jacinta D. Lucero
18The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
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Anup Mahurkar
19Institute for Genomes Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
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Delissa McMillen
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Jeremy A. Miller
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Marmar Moussa
20University of Connecticut, Storrs, CT 06269, USA
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Joseph R. Nery
4Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
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Philip R. Nicovich
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Joshua Orvis
19Institute for Genomes Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
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Julia K. Osteen
18The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
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Scott Owen
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Carter R. Palmer
17Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, USA; Biomedical Sciences Program, School of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
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Thanh Pham
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Nongluk Plongthongkum
3Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
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Olivier Poirion
13Center for Epigenomics, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
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Nora M. Reed
7Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
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Christine Rimorin
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Angeline Rivkin
4Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
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William J. Romanow
21Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, USA
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Adriana E. Sedeño-Cortés
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Kimberly Siletti
22Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
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Saroja Somasundaram
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Josef Sulc
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Michael Tieu
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Amy Torkelson
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Herman Tung
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Xinxin Wang
23McDonnell Genome Institute, Washington University School of Medicine, St. Louis, MO 63108, USA
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Fangming Xie
24Department of Physics, University of California, San Diego, La Jolla, CA 92093, USA
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Anna Marie Yanny
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Renee Zhang
9J. Craig Venter Institute, La Jolla, CA 92037, USA
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Seth A. Ament
19Institute for Genomes Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
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M. Margarita Behrens
18The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
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Hector Corrada Bravo
14Department of Computer Science, University of Maryland College Park, College Park, MD 20742, USA
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Jerold Chun
21Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, USA
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Alexander Dobin
25Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
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Jesse Gillis
6Stanley Institute for Cognitive Genomics, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
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Ronna Hertzano
26Departments of Otorhinolaryngology, Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA; Institute for Genomes Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
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Patrick R. Hof
27Nash Family Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
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Thomas Höllt
28Computer Graphics and Visualization group, Delt University of Technology, the Netherlands
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Gregory D. Horwitz
29Department of Physiology and Biophysics, Washington National Primate Research Center, University of Washington, Seattle, WA 98121, USA
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C. Dirk Keene
30Department of Pathology, University of Washington, Seattle, WA 98195, USA
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Peter V. Kharchenko
31Department of Biomedical Informatics, Harvard Medical School, Boston, MA 02115, USA
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Andrew L. Ko
32Department of Neurological Surgery, University of Washington School of Medicine, Seattle, WA 98195, USA; Regional Epilepsy Center, Harborview Medical Center, Seattle, WA 98104, USA
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Boudewijn P. Lelieveldt
33LKEB, Department of Radiology, Leiden University Medical Center, Leiden, the Netherlands; Pattern Recognition and Bioinformatics group, Delft University of Technology, Delft, the Netherlands
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Chongyuan Luo
34Department of Human Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA
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Eran A. Mukamel
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Sebastian Preissl
13Center for Epigenomics, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
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Aviv Regev
36Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
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Bing Ren
37Center for Epigenomics, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA; Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
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Richard H. Scheuermann
38J. Craig Venter Institute, La Jolla, CA 92037, USA; Department of Pathology, University of California, San Diego, CA 92093, USA; Division of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, CA 92037, USA
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Kimberly Smith
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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William J. Spain
10Epilepsy Center of Excellence, Department of Veteran's Affairs Medical Center, Seattle, WA 98108, USA; Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA
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Owen R. White
19Institute for Genomes Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
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Christof Koch
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Michael Hawrylycz
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Bosiljka Tasic
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Evan Z. Macosko
36Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
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Steven A. McCarroll
39Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
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Jonathan T. Ting
5Allen Institute for Brain Science, Seattle, WA 98109, USA; University of Washington, Department of Physiology and Biophysics, Seattle, WA 98195, USA
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Hongkui Zeng
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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Kun Zhang
3Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
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  • ORCID record for Kun Zhang
Guoping Feng
40McGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
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Joseph R. Ecker
41Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
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Sten Linnarsson
22Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
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Ed S. Lein
1Allen Institute for Brain Science, Seattle, WA 98109, USA
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  • For correspondence: edl@alleninstitute.org trygveb@alleninstitute.org
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Abstract

The primary motor cortex (M1) is essential for voluntary fine motor control and is functionally conserved across mammals. Using high-throughput transcriptomic and epigenomic profiling of over 450,000 single nuclei in human, marmoset monkey, and mouse, we demonstrate a broadly conserved cellular makeup of this region, whose similarity mirrors evolutionary distance and is consistent between the transcriptome and epigenome. The core conserved molecular identity of neuronal and non-neuronal types allowed the generation of a cross-species consensus cell type classification and inference of conserved cell type properties across species. Despite overall conservation, many species specializations were apparent, including differences in cell type proportions, gene expression, DNA methylation, and chromatin state. Few cell type marker genes were conserved across species, providing a short list of candidate genes and regulatory mechanisms responsible for conserved features of homologous cell types, such as the GABAergic chandelier cells. This consensus transcriptomic classification allowed the Patch-seq identification of layer 5 (L5) corticospinal Betz cells in non-human primate and human and characterization of their highly specialized physiology and anatomy. These findings highlight the robust molecular underpinnings of cell type diversity in M1 across mammals and point to the genes and regulatory pathways responsible for the functional identity of cell types and their species-specific adaptations.

Footnotes

  • https://viewer.cytosplore.org/

  • https://biccn.org/data

  • https://nemoarchive.org/

  • https://bioportal.bioontology.org/ontologies/PCL

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Evolution of cellular diversity in primary motor cortex of human, marmoset monkey, and mouse
Trygve E. Bakken, Nikolas L. Jorstad, Qiwen Hu, Blue B. Lake, Wei Tian, Brian E. Kalmbach, Megan Crow, Rebecca D. Hodge, Fenna M. Krienen, Staci A. Sorensen, Jeroen Eggermont, Zizhen Yao, Brian D. Aevermann, Andrew I. Aldridge, Anna Bartlett, Darren Bertagnolli, Tamara Casper, Rosa G. Castanon, Kirsten Crichton, Tanya L. Daigle, Rachel Dalley, Nick Dee, Nikolai Dembrow, Dinh Diep, Song-Lin Ding, Weixiu Dong, Rongxin Fang, Stephan Fischer, Melissa Goldman, Jeff Goldy, Lucas T. Graybuck, Brian R. Herb, Xiaomeng Hou, Jayaram Kancherla, Matthew Kroll, Kanan Lathia, Baldur van Lew, Yang Eric Li, Christine S. Liu, Hanqing Liu, Jacinta D. Lucero, Anup Mahurkar, Delissa McMillen, Jeremy A. Miller, Marmar Moussa, Joseph R. Nery, Philip R. Nicovich, Joshua Orvis, Julia K. Osteen, Scott Owen, Carter R. Palmer, Thanh Pham, Nongluk Plongthongkum, Olivier Poirion, Nora M. Reed, Christine Rimorin, Angeline Rivkin, William J. Romanow, Adriana E. Sedeño-Cortés, Kimberly Siletti, Saroja Somasundaram, Josef Sulc, Michael Tieu, Amy Torkelson, Herman Tung, Xinxin Wang, Fangming Xie, Anna Marie Yanny, Renee Zhang, Seth A. Ament, M. Margarita Behrens, Hector Corrada Bravo, Jerold Chun, Alexander Dobin, Jesse Gillis, Ronna Hertzano, Patrick R. Hof, Thomas Höllt, Gregory D. Horwitz, C. Dirk Keene, Peter V. Kharchenko, Andrew L. Ko, Boudewijn P. Lelieveldt, Chongyuan Luo, Eran A. Mukamel, Sebastian Preissl, Aviv Regev, Bing Ren, Richard H. Scheuermann, Kimberly Smith, William J. Spain, Owen R. White, Christof Koch, Michael Hawrylycz, Bosiljka Tasic, Evan Z. Macosko, Steven A. McCarroll, Jonathan T. Ting, Hongkui Zeng, Kun Zhang, Guoping Feng, Joseph R. Ecker, Sten Linnarsson, Ed S. Lein
bioRxiv 2020.03.31.016972; doi: https://doi.org/10.1101/2020.03.31.016972
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Evolution of cellular diversity in primary motor cortex of human, marmoset monkey, and mouse
Trygve E. Bakken, Nikolas L. Jorstad, Qiwen Hu, Blue B. Lake, Wei Tian, Brian E. Kalmbach, Megan Crow, Rebecca D. Hodge, Fenna M. Krienen, Staci A. Sorensen, Jeroen Eggermont, Zizhen Yao, Brian D. Aevermann, Andrew I. Aldridge, Anna Bartlett, Darren Bertagnolli, Tamara Casper, Rosa G. Castanon, Kirsten Crichton, Tanya L. Daigle, Rachel Dalley, Nick Dee, Nikolai Dembrow, Dinh Diep, Song-Lin Ding, Weixiu Dong, Rongxin Fang, Stephan Fischer, Melissa Goldman, Jeff Goldy, Lucas T. Graybuck, Brian R. Herb, Xiaomeng Hou, Jayaram Kancherla, Matthew Kroll, Kanan Lathia, Baldur van Lew, Yang Eric Li, Christine S. Liu, Hanqing Liu, Jacinta D. Lucero, Anup Mahurkar, Delissa McMillen, Jeremy A. Miller, Marmar Moussa, Joseph R. Nery, Philip R. Nicovich, Joshua Orvis, Julia K. Osteen, Scott Owen, Carter R. Palmer, Thanh Pham, Nongluk Plongthongkum, Olivier Poirion, Nora M. Reed, Christine Rimorin, Angeline Rivkin, William J. Romanow, Adriana E. Sedeño-Cortés, Kimberly Siletti, Saroja Somasundaram, Josef Sulc, Michael Tieu, Amy Torkelson, Herman Tung, Xinxin Wang, Fangming Xie, Anna Marie Yanny, Renee Zhang, Seth A. Ament, M. Margarita Behrens, Hector Corrada Bravo, Jerold Chun, Alexander Dobin, Jesse Gillis, Ronna Hertzano, Patrick R. Hof, Thomas Höllt, Gregory D. Horwitz, C. Dirk Keene, Peter V. Kharchenko, Andrew L. Ko, Boudewijn P. Lelieveldt, Chongyuan Luo, Eran A. Mukamel, Sebastian Preissl, Aviv Regev, Bing Ren, Richard H. Scheuermann, Kimberly Smith, William J. Spain, Owen R. White, Christof Koch, Michael Hawrylycz, Bosiljka Tasic, Evan Z. Macosko, Steven A. McCarroll, Jonathan T. Ting, Hongkui Zeng, Kun Zhang, Guoping Feng, Joseph R. Ecker, Sten Linnarsson, Ed S. Lein
bioRxiv 2020.03.31.016972; doi: https://doi.org/10.1101/2020.03.31.016972

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