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Early-life hypoxia alters adult physiology and reduces stress resistance and lifespan in Drosophila

Danielle M Polan, Mohammad Alansari, Byoungchun Lee, View ORCID ProfileSavraj Grewal
doi: https://doi.org/10.1101/2020.03.31.018960
Danielle M Polan
1Clark H Smith Brain Tumour Centre, Arnie Charbonneau Cancer Institute, Alberta Children’s Hospital Research Institute, and Department of Biochemistry and Molecular Biology Calgary, University of Calgary, Alberta T2N 4N1, Canada
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Mohammad Alansari
1Clark H Smith Brain Tumour Centre, Arnie Charbonneau Cancer Institute, Alberta Children’s Hospital Research Institute, and Department of Biochemistry and Molecular Biology Calgary, University of Calgary, Alberta T2N 4N1, Canada
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Byoungchun Lee
1Clark H Smith Brain Tumour Centre, Arnie Charbonneau Cancer Institute, Alberta Children’s Hospital Research Institute, and Department of Biochemistry and Molecular Biology Calgary, University of Calgary, Alberta T2N 4N1, Canada
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Savraj Grewal
1Clark H Smith Brain Tumour Centre, Arnie Charbonneau Cancer Institute, Alberta Children’s Hospital Research Institute, and Department of Biochemistry and Molecular Biology Calgary, University of Calgary, Alberta T2N 4N1, Canada
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  • ORCID record for Savraj Grewal
  • For correspondence: grewalss@ucalgary.ca
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ABSTRACT

In many animals, short-term fluctuations in environmental conditions in early life often exert long-term effects on adult physiology. In Drosophila, one ecologically relevant environmental variable is hypoxia. Drosophila larvae live on rotting, fermenting food rich in microorganisms – an environment characterized by low ambient oxygen. They have therefore evolved to tolerate hypoxia. While the acute effects of hypoxia in larvae have been well studied, whether early-life hypoxia affects adult physiology and fitness is less clear. Here we show that Drosophila exposed to hypoxia during their larval period subsequently show reduced starvation stress resistance and shorter lifespan as adults, with these effects being stronger in males. We find that these effects are associated with reduced whole-body insulin signaling but elevated TOR kinase activity, a manipulation known to reduce lifespan. We also identify a sexually dimorphic effect of larval hypoxia on adult nutrient storage and mobilization. Thus, we find that males, but not females, showing elevated levels of lipids and glycogen. Moreover, we see that both males and females exposed to hypoxia as larvae show defective lipid mobilization upon starvation stress as adults. These data show how early-life hypoxia can exert persistent, sexually dimorphic, long-term effects on Drosophila adult physiology and lifespan.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted April 01, 2020.
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Early-life hypoxia alters adult physiology and reduces stress resistance and lifespan in Drosophila
Danielle M Polan, Mohammad Alansari, Byoungchun Lee, Savraj Grewal
bioRxiv 2020.03.31.018960; doi: https://doi.org/10.1101/2020.03.31.018960
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Early-life hypoxia alters adult physiology and reduces stress resistance and lifespan in Drosophila
Danielle M Polan, Mohammad Alansari, Byoungchun Lee, Savraj Grewal
bioRxiv 2020.03.31.018960; doi: https://doi.org/10.1101/2020.03.31.018960

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