Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Pod indehiscence in common bean is associated to the fine regulation of PvMYB26 and a non-functional abscission layer

Valerio Di Vittori, Elena Bitocchi, Monica Rodriguez, View ORCID ProfileSaleh Alseekh, Elisa Bellucci, Laura Nanni, View ORCID ProfileTania Gioia, Stefania Marzario, Giuseppina Logozzo, Marzia Rossato, Concetta De Quattro, Maria L. Murgia, View ORCID ProfileJuan José Ferreira, Ana Campa, Chunming Xu, View ORCID ProfileFabio Fiorani, View ORCID ProfileArun Sampathkumar, Anja Fröhlich, Giovanna Attene, View ORCID ProfileMassimo Delledonne, Björn Usadel, View ORCID ProfileAlisdair R. Fernie, Domenico Rau, View ORCID ProfileRoberto Papa
doi: https://doi.org/10.1101/2020.04.02.021972
Valerio Di Vittori
aDipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elena Bitocchi
aDipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Monica Rodriguez
bDipartimento di Agraria, Università degli Studi di Sassari, Via E. De Nicola, 07100 Sassari, Italy
cCentro per la Conservazione e Valorizzazione della Biodiversità Vegetale, Università degli Studi di Sassari, SS 127bis, km 28.500 Surigheddu, 07041 Alghero, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Saleh Alseekh
dMax-Planck-Institute of Molecular Plant Physiology, Am Müehlenberg 1, Potsdam-Golm, 14476 Germany
eCenter of Plant Systems Biology and Biotechnology, 4000 Plovdiv, Bulgaria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Saleh Alseekh
Elisa Bellucci
aDipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Laura Nanni
aDipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tania Gioia
fScuola di Scienze Agrarie, Forestali, Alimentari ed Ambientali, Università degli Studi della Basilicata, viale dell’Ateneo Lucano 10, 85100 Potenza, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Tania Gioia
Stefania Marzario
fScuola di Scienze Agrarie, Forestali, Alimentari ed Ambientali, Università degli Studi della Basilicata, viale dell’Ateneo Lucano 10, 85100 Potenza, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Giuseppina Logozzo
fScuola di Scienze Agrarie, Forestali, Alimentari ed Ambientali, Università degli Studi della Basilicata, viale dell’Ateneo Lucano 10, 85100 Potenza, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marzia Rossato
gDipartimento di Biotecnologie, Università degli Studi di Verona, Cà Vignal 1, Strada Le Grazie 15, 37134 Verona, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Concetta De Quattro
gDipartimento di Biotecnologie, Università degli Studi di Verona, Cà Vignal 1, Strada Le Grazie 15, 37134 Verona, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maria L. Murgia
bDipartimento di Agraria, Università degli Studi di Sassari, Via E. De Nicola, 07100 Sassari, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Juan José Ferreira
hPlant Genetics Group, Agri-Food Research and Development Regional Service (SERIDA), 33300, Asturias, Spain
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Juan José Ferreira
Ana Campa
hPlant Genetics Group, Agri-Food Research and Development Regional Service (SERIDA), 33300, Asturias, Spain
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chunming Xu
iKey Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun, 130024 China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fabio Fiorani
jInstitute of Biosciences and Geosciences (IBG-2): Plant Sciences, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Fabio Fiorani
Arun Sampathkumar
dMax-Planck-Institute of Molecular Plant Physiology, Am Müehlenberg 1, Potsdam-Golm, 14476 Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Arun Sampathkumar
Anja Fröhlich
dMax-Planck-Institute of Molecular Plant Physiology, Am Müehlenberg 1, Potsdam-Golm, 14476 Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Giovanna Attene
bDipartimento di Agraria, Università degli Studi di Sassari, Via E. De Nicola, 07100 Sassari, Italy
cCentro per la Conservazione e Valorizzazione della Biodiversità Vegetale, Università degli Studi di Sassari, SS 127bis, km 28.500 Surigheddu, 07041 Alghero, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Massimo Delledonne
gDipartimento di Biotecnologie, Università degli Studi di Verona, Cà Vignal 1, Strada Le Grazie 15, 37134 Verona, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Massimo Delledonne
Björn Usadel
jInstitute of Biosciences and Geosciences (IBG-2): Plant Sciences, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alisdair R. Fernie
dMax-Planck-Institute of Molecular Plant Physiology, Am Müehlenberg 1, Potsdam-Golm, 14476 Germany
eCenter of Plant Systems Biology and Biotechnology, 4000 Plovdiv, Bulgaria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Alisdair R. Fernie
Domenico Rau
bDipartimento di Agraria, Università degli Studi di Sassari, Via E. De Nicola, 07100 Sassari, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Roberto Papa
aDipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Roberto Papa
  • For correspondence: r.papa@univpm.it
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

ABSTRACT

In legumes, pod shattering occurs when mature pods dehisce along the sutures, and detachment of the valves promotes seed dispersal. In Phaseolus vulgaris, the major locus qPD5.1-Pv for pod indehiscence was identified recently. We developed a BC4/F4 introgression line population and narrowed the major locus down to a 22.5-kb region. Here, gene expression and a parallel histological analysis of dehiscent and indehiscent pods identified an AtMYB26 orthologue as the best candidate for loss of pod shattering, on a genomic region ~11 kb downstream of the highest associated peak. Based on mapping and expression data, we propose early and fine up-regulation of PvMYB26 in dehiscent pods. Detailed histological analysis establishes that pod indehiscence is associated to the lack of a functional abscission layer in the ventral sheath, and that the key anatomical modifications associated with pod shattering in common bean occur early during pod development. We finally propose that loss of pod shattering in legumes resulted from histological convergent evolution and that this is the result of selection at orthologous loci.

One-sentence summary A non-functional abscission layer determines the loss of pod shattering; mapping data, and parallel gene expression and histological analysis support PvMYB26 as the candidate gene for pod indehiscence.

Footnotes

  • The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Roberto Papa (r.papa{at}univpm.it).

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted April 04, 2020.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Pod indehiscence in common bean is associated to the fine regulation of PvMYB26 and a non-functional abscission layer
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Pod indehiscence in common bean is associated to the fine regulation of PvMYB26 and a non-functional abscission layer
Valerio Di Vittori, Elena Bitocchi, Monica Rodriguez, Saleh Alseekh, Elisa Bellucci, Laura Nanni, Tania Gioia, Stefania Marzario, Giuseppina Logozzo, Marzia Rossato, Concetta De Quattro, Maria L. Murgia, Juan José Ferreira, Ana Campa, Chunming Xu, Fabio Fiorani, Arun Sampathkumar, Anja Fröhlich, Giovanna Attene, Massimo Delledonne, Björn Usadel, Alisdair R. Fernie, Domenico Rau, Roberto Papa
bioRxiv 2020.04.02.021972; doi: https://doi.org/10.1101/2020.04.02.021972
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Pod indehiscence in common bean is associated to the fine regulation of PvMYB26 and a non-functional abscission layer
Valerio Di Vittori, Elena Bitocchi, Monica Rodriguez, Saleh Alseekh, Elisa Bellucci, Laura Nanni, Tania Gioia, Stefania Marzario, Giuseppina Logozzo, Marzia Rossato, Concetta De Quattro, Maria L. Murgia, Juan José Ferreira, Ana Campa, Chunming Xu, Fabio Fiorani, Arun Sampathkumar, Anja Fröhlich, Giovanna Attene, Massimo Delledonne, Björn Usadel, Alisdair R. Fernie, Domenico Rau, Roberto Papa
bioRxiv 2020.04.02.021972; doi: https://doi.org/10.1101/2020.04.02.021972

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genetics
Subject Areas
All Articles
  • Animal Behavior and Cognition (3505)
  • Biochemistry (7348)
  • Bioengineering (5324)
  • Bioinformatics (20266)
  • Biophysics (10019)
  • Cancer Biology (7744)
  • Cell Biology (11305)
  • Clinical Trials (138)
  • Developmental Biology (6437)
  • Ecology (9953)
  • Epidemiology (2065)
  • Evolutionary Biology (13325)
  • Genetics (9361)
  • Genomics (12586)
  • Immunology (7702)
  • Microbiology (19024)
  • Molecular Biology (7443)
  • Neuroscience (41041)
  • Paleontology (300)
  • Pathology (1229)
  • Pharmacology and Toxicology (2138)
  • Physiology (3161)
  • Plant Biology (6861)
  • Scientific Communication and Education (1273)
  • Synthetic Biology (1896)
  • Systems Biology (5313)
  • Zoology (1089)