Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

The Arabidopsis NOT4A E3 ligase coordinates PGR3 expression to regulate chloroplast protein translation

View ORCID ProfileMark Bailey, View ORCID ProfileAiste Ivanauskaite, Julia Grimmer, Oluwatunmise Akintewe, Adrienne C. Payne, Ross Etherington, View ORCID ProfileAnne-Marie Labandera, Rory Osborne, View ORCID ProfileMarjaana Rantala, View ORCID ProfileSacha Baginsky, View ORCID ProfilePaula Mulo, View ORCID ProfileDaniel J. Gibbs
doi: https://doi.org/10.1101/2020.04.02.021998
Mark Bailey
1School of Biosciences, University of Birmingham, Edgbaston, UK, B15 2TT
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mark Bailey
Aiste Ivanauskaite
2Molecular Plant Biology, University of Turku, 20520 Turku, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Aiste Ivanauskaite
Julia Grimmer
3Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 3a, 06120 Halle (Saale), Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Oluwatunmise Akintewe
1School of Biosciences, University of Birmingham, Edgbaston, UK, B15 2TT
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Adrienne C. Payne
1School of Biosciences, University of Birmingham, Edgbaston, UK, B15 2TT
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ross Etherington
1School of Biosciences, University of Birmingham, Edgbaston, UK, B15 2TT
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anne-Marie Labandera
1School of Biosciences, University of Birmingham, Edgbaston, UK, B15 2TT
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Anne-Marie Labandera
Rory Osborne
1School of Biosciences, University of Birmingham, Edgbaston, UK, B15 2TT
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marjaana Rantala
2Molecular Plant Biology, University of Turku, 20520 Turku, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Marjaana Rantala
Sacha Baginsky
3Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 3a, 06120 Halle (Saale), Germany
4Biochemistry of Plants, Faculty for Biology and Biotechnology, Ruhr-University Bochum, Universitätsstrasse 150, 44801 Bochum, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sacha Baginsky
Paula Mulo
2Molecular Plant Biology, University of Turku, 20520 Turku, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Paula Mulo
Daniel J. Gibbs
1School of Biosciences, University of Birmingham, Edgbaston, UK, B15 2TT
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Daniel J. Gibbs
  • For correspondence: d.gibbs@bham.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Chloroplast function requires the coordinated action of nuclear- and chloroplast-derived proteins, including several hundred nuclear-encoded pentatricopeptide repeat (PPR) proteins that regulate plastid mRNA metabolism. Despite their large number and importance, regulatory mechanisms controlling PPR expression are poorly understood. Here we show that the Arabidopsis NOT4A ubiquitin-ligase positively regulates PROTON GRADIENT 3 (PGR3), a PPR protein required for translating 30S ribosome subunits and several thylakoid-localised photosynthetic components within chloroplasts. Loss of NOT4A function leads to a strong depletion of plastid ribosomes, which reduces mRNA translation and negatively impacts photosynthetic capacity, causing pale-yellow and slow-growth phenotypes. Quantitative transcriptome and proteome analyses reveal that these defects are due to a lack of PGR3 expression in not4a, and we show that normal plastid function is restored through transgenic PGR3 expression. Our work identifies NOT4A as crucial for ensuring robust photosynthetic function during development and stress-response, through modulating PGR3 levels to coordinate chloroplast protein synthesis.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted April 03, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
The Arabidopsis NOT4A E3 ligase coordinates PGR3 expression to regulate chloroplast protein translation
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
The Arabidopsis NOT4A E3 ligase coordinates PGR3 expression to regulate chloroplast protein translation
Mark Bailey, Aiste Ivanauskaite, Julia Grimmer, Oluwatunmise Akintewe, Adrienne C. Payne, Ross Etherington, Anne-Marie Labandera, Rory Osborne, Marjaana Rantala, Sacha Baginsky, Paula Mulo, Daniel J. Gibbs
bioRxiv 2020.04.02.021998; doi: https://doi.org/10.1101/2020.04.02.021998
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
The Arabidopsis NOT4A E3 ligase coordinates PGR3 expression to regulate chloroplast protein translation
Mark Bailey, Aiste Ivanauskaite, Julia Grimmer, Oluwatunmise Akintewe, Adrienne C. Payne, Ross Etherington, Anne-Marie Labandera, Rory Osborne, Marjaana Rantala, Sacha Baginsky, Paula Mulo, Daniel J. Gibbs
bioRxiv 2020.04.02.021998; doi: https://doi.org/10.1101/2020.04.02.021998

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Plant Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4109)
  • Biochemistry (8813)
  • Bioengineering (6517)
  • Bioinformatics (23456)
  • Biophysics (11788)
  • Cancer Biology (9205)
  • Cell Biology (13318)
  • Clinical Trials (138)
  • Developmental Biology (7433)
  • Ecology (11407)
  • Epidemiology (2066)
  • Evolutionary Biology (15145)
  • Genetics (10433)
  • Genomics (14041)
  • Immunology (9169)
  • Microbiology (22152)
  • Molecular Biology (8808)
  • Neuroscience (47558)
  • Paleontology (350)
  • Pathology (1428)
  • Pharmacology and Toxicology (2491)
  • Physiology (3730)
  • Plant Biology (8079)
  • Scientific Communication and Education (1437)
  • Synthetic Biology (2220)
  • Systems Biology (6037)
  • Zoology (1252)