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Robust neutralization assay based on SARS-CoV-2 S-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressed BHK21 cells
Hua-Long Xiong, Yang-Tao Wu, Jia-Li Cao, Ren Yang, Jian Ma, Xiao-Yang Qiao, Xiang-Yang Yao, Bao-Hui Zhang, Ya-Li Zhang, Wang-Heng Hou, Yang-Shi, Jing-Jing Xu, Liang-Zhang, Shao-Juan Wang, Bao-Rong Fu, Ting Yang, Sheng-Xiang Ge, Jun Zhang, Quan Yuan, Bao-Ying Huang, Zhi-Yong Li, Tian-Ying Zhang, View ORCID ProfileNing-Shao Xia
doi: https://doi.org/10.1101/2020.04.08.026948
Hua-Long Xiong
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Yang-Tao Wu
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Jia-Li Cao
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Ren Yang
2NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China
Jian Ma
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Xiao-Yang Qiao
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Xiang-Yang Yao
3The First Hospital of Xiamen University, Xiamen 361003, China
Bao-Hui Zhang
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Ya-Li Zhang
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Wang-Heng Hou
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Yang-Shi
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Jing-Jing Xu
4Department of Hematology, Fujian Medical University Union Hospital, Fujian Provincial Key Laboratory on Hematology, Fujian Institute of Hematology, Fuzhou 350000, China
Liang-Zhang
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Shao-Juan Wang
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Bao-Rong Fu
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Ting Yang
4Department of Hematology, Fujian Medical University Union Hospital, Fujian Provincial Key Laboratory on Hematology, Fujian Institute of Hematology, Fuzhou 350000, China
Sheng-Xiang Ge
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Jun Zhang
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Quan Yuan
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Bao-Ying Huang
2NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China
Zhi-Yong Li
3The First Hospital of Xiamen University, Xiamen 361003, China
Tian-Ying Zhang
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Ning-Shao Xia
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health and School of Life Sciences, Xiamen University, Xiamen 361102, China
Posted April 13, 2020.
Robust neutralization assay based on SARS-CoV-2 S-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressed BHK21 cells
Hua-Long Xiong, Yang-Tao Wu, Jia-Li Cao, Ren Yang, Jian Ma, Xiao-Yang Qiao, Xiang-Yang Yao, Bao-Hui Zhang, Ya-Li Zhang, Wang-Heng Hou, Yang-Shi, Jing-Jing Xu, Liang-Zhang, Shao-Juan Wang, Bao-Rong Fu, Ting Yang, Sheng-Xiang Ge, Jun Zhang, Quan Yuan, Bao-Ying Huang, Zhi-Yong Li, Tian-Ying Zhang, Ning-Shao Xia
bioRxiv 2020.04.08.026948; doi: https://doi.org/10.1101/2020.04.08.026948
Robust neutralization assay based on SARS-CoV-2 S-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressed BHK21 cells
Hua-Long Xiong, Yang-Tao Wu, Jia-Li Cao, Ren Yang, Jian Ma, Xiao-Yang Qiao, Xiang-Yang Yao, Bao-Hui Zhang, Ya-Li Zhang, Wang-Heng Hou, Yang-Shi, Jing-Jing Xu, Liang-Zhang, Shao-Juan Wang, Bao-Rong Fu, Ting Yang, Sheng-Xiang Ge, Jun Zhang, Quan Yuan, Bao-Ying Huang, Zhi-Yong Li, Tian-Ying Zhang, Ning-Shao Xia
bioRxiv 2020.04.08.026948; doi: https://doi.org/10.1101/2020.04.08.026948
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