Abstract
Clinical samples collected in COVID-19 patients are commonly manipulated in BSL-2 laboratories for diagnostic purpose. We used the French norm NF-EN-14476+A2 derived from the European standard EN-14885. To avoid the risk of exposure of laboratory workers, we showed that Sodium-dodecyl-sulfate and Triton-X100 must be added to guanidinium thiocyanate-lysis buffers to obtain a 6-log reduction of infectious virus. Heating protocol consisting of 92°C-15min was more effective rather than 56°C-30min and 60°C-60min to achieve 6-log reduction.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
* Funding Statement, This study was partially funded by (i) the “European Virus Archive Global” (EVA-GLOBAL) project H2020-INFRAIA-2019 program, Project No 871029, (ii) the “Advanced Nanosensing platforms for Point of care glovbal disgnostics and surveillance” (CONVAT), H2020, Project No101003544, (iii) “Preparedness and Response in an Emergency contact to Pathogens of Medical and Veterinary importance” (PREPMedVet), Agence Nationale de la Recherche Franco-German call on Civil security / Global security 2019 Edition, (iv) “Viral Hemorrhagic fever modern approaches for developing bedside rapid diagnostics” (VHFModRAD), IMI2 Program, H2020, Project No823666, and the Inserm through the Reacting (REsearch and ACTion Targeting emerging infectious diseases) initiative.
