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Isolation, sequence, infectivity and replication kinetics of SARS-CoV-2

View ORCID ProfileArinjay Banerjee, Jalees A. Nasir, Patrick Budylowski, Lily Yip, Patryk Aftanas, Natasha Christie, Ayoob Ghalami, Kaushal Baid, Amogelang R. Raphenya, Jeremy A. Hirota, Matthew S. Miller, Allison J. McGeer, Mario Ostrowski, Robert A. Kozak, Andrew G. McArthur, Karen Mossman, Samira Mubareka
doi: https://doi.org/10.1101/2020.04.11.037382
Arinjay Banerjee
1McMaster University, Hamilton, Ontario, Canada
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  • ORCID record for Arinjay Banerjee
Jalees A. Nasir
1McMaster University, Hamilton, Ontario, Canada
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Patrick Budylowski
2University of Toronto, Toronto, Ontario, Canada
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Lily Yip
3Sunnybrook Research Institute, Toronto, Ontario, Canada
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Patryk Aftanas
3Sunnybrook Research Institute, Toronto, Ontario, Canada
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Natasha Christie
2University of Toronto, Toronto, Ontario, Canada
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Ayoob Ghalami
2University of Toronto, Toronto, Ontario, Canada
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Kaushal Baid
1McMaster University, Hamilton, Ontario, Canada
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Amogelang R. Raphenya
1McMaster University, Hamilton, Ontario, Canada
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Jeremy A. Hirota
1McMaster University, Hamilton, Ontario, Canada
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Matthew S. Miller
1McMaster University, Hamilton, Ontario, Canada
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Allison J. McGeer
2University of Toronto, Toronto, Ontario, Canada
4Mount Sinai Hospital, Toronto, Ontario, Canada
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Mario Ostrowski
2University of Toronto, Toronto, Ontario, Canada
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Robert A. Kozak
2University of Toronto, Toronto, Ontario, Canada
3Sunnybrook Research Institute, Toronto, Ontario, Canada
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Andrew G. McArthur
1McMaster University, Hamilton, Ontario, Canada
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Karen Mossman
1McMaster University, Hamilton, Ontario, Canada
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  • For correspondence: mossk@mcmaster.ca
Samira Mubareka
2University of Toronto, Toronto, Ontario, Canada
3Sunnybrook Research Institute, Toronto, Ontario, Canada
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ABSTRACT

SARS-CoV-2 emerged in December 2019 in Wuhan, China and has since infected over 1.5 million people, of which over 107,000 have died. As SARS-CoV-2 spreads across the planet, speculations remain about the range of human cells that can be infected by SARS-CoV-2. In this study, we report the isolation of SARS-CoV-2 from two cases of COVID-19 in Toronto, Canada. We determined the genomic sequences of the two isolates and identified single nucleotide changes in representative populations of our virus stocks. More importantly, we tested a wide range of human immune cells for productive infection with SARS-CoV-2. Here we confirm that human primary peripheral blood mononuclear cells (PBMCs) are not permissive for SARS-CoV-2. As SARS-CoV-2 continues to spread globally, it is essential to monitor single nucleotide polymorphisms in the virus and to continue to isolate circulating viruses to determine viral genotype and phenotype using in vitro and in vivo infection models.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Discussion has been expanded and minor typos have been addressed. The title has been shortened.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted April 12, 2020.
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Isolation, sequence, infectivity and replication kinetics of SARS-CoV-2
Arinjay Banerjee, Jalees A. Nasir, Patrick Budylowski, Lily Yip, Patryk Aftanas, Natasha Christie, Ayoob Ghalami, Kaushal Baid, Amogelang R. Raphenya, Jeremy A. Hirota, Matthew S. Miller, Allison J. McGeer, Mario Ostrowski, Robert A. Kozak, Andrew G. McArthur, Karen Mossman, Samira Mubareka
bioRxiv 2020.04.11.037382; doi: https://doi.org/10.1101/2020.04.11.037382
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Isolation, sequence, infectivity and replication kinetics of SARS-CoV-2
Arinjay Banerjee, Jalees A. Nasir, Patrick Budylowski, Lily Yip, Patryk Aftanas, Natasha Christie, Ayoob Ghalami, Kaushal Baid, Amogelang R. Raphenya, Jeremy A. Hirota, Matthew S. Miller, Allison J. McGeer, Mario Ostrowski, Robert A. Kozak, Andrew G. McArthur, Karen Mossman, Samira Mubareka
bioRxiv 2020.04.11.037382; doi: https://doi.org/10.1101/2020.04.11.037382

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