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History
- April 15, 2020.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Author Information
- Michael Jakob Pichler1,#,
- Chihaya Yamada2,#,
- Bashar Shuoker1,7,
- Maria Camila Alvarez-Silva6,
- Aina Gotoh3,
- Maria Louise Leth1,
- Erwin Schoof1,
- Toshihiko Katoh3,
- Mikiyasu Sakanaka4,
- Takane Katayama3,4,
- Chunsheng Jin5,
- Niclas G. Karlsson5,
- Manimozhiyan Arumugam6,
- Shinya Fushinobu2 and
- Maher Abou Hachem1,*
- 1Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, 2800, Denmark
- 2Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan
- 3Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan
- 4Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, Ishikawa, 921-8836, Japan
- 5Department of Medical Biochemistry, University of Gothenburg, Gothenburg, 413 90, Sweden
- 6The Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, 2200, Denmark
- 7Division of Biotechnology, Department of Chemistry, Lund University, Lund, 22100, Sweden
- ↵*Correspondence: maha{at}bio.dtu.dk
↵# These authors contributed equally to this work
Contributions, Bacterial growth studies were performed by M.J.P. Proteomic analyses were done by M.J.P and E.S. Protein characterization was done by M.J.P and M.L.L. Enzymatic characterization of RiLea/b136 was performed by A.G.,To.K., M.S. and Ta.K. Mucin preparation was performed by B.S. and M.J.P. Mucin glycomics were performed by B.S., C.J. and N.G.K. Protein X-ray crystallography was performed by C.Y. and S.F. Metagenome analysis were performed by C.A-S and M.A. Experiments were designed by M.J.P and M.A.H. The manuscript was drafted by M.J.P and M.A.H and finalized with contributions of all authors.