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A multivalent fuzzy interface drives reversible COPII coat assembly

Viktoriya G. Stancheva, Joshua Hutchings, Xiao-Han Li, Balaji Santhanam, M. Madan Babu, Giulia Zanetti, View ORCID ProfileElizabeth A. Miller
doi: https://doi.org/10.1101/2020.04.15.043356
Viktoriya G. Stancheva
1MRC Laboratory of Molecular Biology
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Joshua Hutchings
2Institute of Structural and Molecular Biology, Birkbeck College
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Xiao-Han Li
1MRC Laboratory of Molecular Biology
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Balaji Santhanam
1MRC Laboratory of Molecular Biology
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M. Madan Babu
1MRC Laboratory of Molecular Biology
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Giulia Zanetti
2Institute of Structural and Molecular Biology, Birkbeck College
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Elizabeth A. Miller
1MRC Laboratory of Molecular Biology
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  • ORCID record for Elizabeth A. Miller
  • For correspondence: emiller@mrc-lmb.cam.ac.uk
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Abstract

Protein secretion is initiated at the endoplasmic reticulum by the COPII coat, which self-assembles to form vesicles. Here, we examine the mechanisms by which the outer scaffolding layer of the coat drives local assembly of a structure rigid enough to enforce membrane curvature, yet able to readily disassemble at the Golgi. An intrinsically disordered region in the outer coat protein, Sec31, drives binding with an inner coat layer via multiple distinct interfaces. Interactions are individually dispensable but combinatorially reinforce each other, suggesting coat oligomerization is driven by the cumulative effects of multivalent interactions. Such a multimodal assembly platform could be readily reversed at the Golgi via perturbation of each individual interface. These design principles provide an explanation for how cells build a powerful yet transient scaffold to direct vesicle traffic.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted April 16, 2020.
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A multivalent fuzzy interface drives reversible COPII coat assembly
Viktoriya G. Stancheva, Joshua Hutchings, Xiao-Han Li, Balaji Santhanam, M. Madan Babu, Giulia Zanetti, Elizabeth A. Miller
bioRxiv 2020.04.15.043356; doi: https://doi.org/10.1101/2020.04.15.043356
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A multivalent fuzzy interface drives reversible COPII coat assembly
Viktoriya G. Stancheva, Joshua Hutchings, Xiao-Han Li, Balaji Santhanam, M. Madan Babu, Giulia Zanetti, Elizabeth A. Miller
bioRxiv 2020.04.15.043356; doi: https://doi.org/10.1101/2020.04.15.043356

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