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Functional MRI of large scale activity in behaving mice

View ORCID ProfileMadalena S. Fonseca, View ORCID ProfileMattia G. Bergomi, View ORCID ProfileZachary F. Mainen, View ORCID ProfileNoam Shemesh
doi: https://doi.org/10.1101/2020.04.16.044941
Madalena S. Fonseca
Champalimaud Research, Champalimaud Centre for the Unknown, Lisbon, Portugal
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  • ORCID record for Madalena S. Fonseca
  • For correspondence: madalena.fonseca@neuro.fchampalimaud.org noam.shemesh@neuro.fchampalimaud.org
Mattia G. Bergomi
Champalimaud Research, Champalimaud Centre for the Unknown, Lisbon, Portugal
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  • ORCID record for Mattia G. Bergomi
Zachary F. Mainen
Champalimaud Research, Champalimaud Centre for the Unknown, Lisbon, Portugal
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Noam Shemesh
Champalimaud Research, Champalimaud Centre for the Unknown, Lisbon, Portugal
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  • For correspondence: madalena.fonseca@neuro.fchampalimaud.org noam.shemesh@neuro.fchampalimaud.org
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ABSTRACT

Behaviour involves complex dynamic interactions across many brain regions. Detecting whole-brain activity in mice performing sophisticated behavioural tasks could facilitate insights into distributed processing underlying behaviour, guide local targeting, and help bridge the disparate spatial scales between rodent and human studies. Here, we present a comprehensive approach for recording brain-wide activity with functional magnetic resonance imaging (fMRI) compatible with a wide range of behavioural paradigms and neuroscience questions. We introduce hardware and procedural advances to allow multi-sensory, multi-action behavioural paradigms in the scanner. We identify signal artefacts arising from task-related body movements and propose novel strategies to reduce them. We validate and explore our approach in a 4-odour classical conditioning and a visually-guided operant task, illustrating how it can be used to extract information so far inaccessible to rodent behaviour studies. Our work paves the way for future studies combining fMRI and local circuit techniques during complex behaviour to tackle multi-scale behavioural neuroscience questions.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 17, 2022.
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Functional MRI of large scale activity in behaving mice
Madalena S. Fonseca, Mattia G. Bergomi, Zachary F. Mainen, Noam Shemesh
bioRxiv 2020.04.16.044941; doi: https://doi.org/10.1101/2020.04.16.044941
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Functional MRI of large scale activity in behaving mice
Madalena S. Fonseca, Mattia G. Bergomi, Zachary F. Mainen, Noam Shemesh
bioRxiv 2020.04.16.044941; doi: https://doi.org/10.1101/2020.04.16.044941

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