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Species-specific quorum sensing represses the chitobiose utilization locus in Vibrio cholerae

Catherine A. Klancher, Jane D. Newman, Alyssa S. Ball, Julia C. van Kessel, Ankur B. Dalia
doi: https://doi.org/10.1101/2020.04.16.045526
Catherine A. Klancher
Department of Biology, Indiana University, Bloomington, IN 47405
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Jane D. Newman
Department of Biology, Indiana University, Bloomington, IN 47405
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Alyssa S. Ball
Department of Biology, Indiana University, Bloomington, IN 47405
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Julia C. van Kessel
Department of Biology, Indiana University, Bloomington, IN 47405
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Ankur B. Dalia
Department of Biology, Indiana University, Bloomington, IN 47405
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  • For correspondence: ankdalia@indiana.edu
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Abstract

The marine facultative pathogen Vibrio cholerae forms complex multicellular communities on the chitinous shells of crustacean zooplankton in its aquatic reservoir. V. cholerae-chitin interactions are critical for the growth, evolution, and waterborne transmission of cholera. This is due, in part, to chitin-induced changes in gene expression in this pathogen. Here, we sought to identify factors that influence chitin-induced expression of one locus, the chitobiose utilization operon (chb), which is required for the uptake and catabolism of the chitin disaccharide. Through a series of genetic screens, we identified that the master regulator of quorum sensing, HapR, is a direct repressor of the chb operon. We also found that the levels of HapR in V. cholerae are regulated by the ClpAP protease. Furthermore, we show that the canonical quorum sensing cascade in V. cholerae regulates chb expression in a HapR-dependent manner. Through this analysis we found that signaling via the species-specific autoinducer CAI-1, but not the inter-species autoinducer AI-2, influences chb expression. This phenomenon of species-specific regulation may enhance the fitness of this pathogen in its environmental niche.

Importance In nature, bacteria live in multicellular and multispecies communities. Microbial species can sense the density and composition of their community through chemical cues using a process called quorum sensing (QS). The marine pathogen Vibrio cholerae is found in communities on the chitinous shells of crustaceans in its aquatic reservoir. V. cholerae interactions with chitin are critical for the survival, evolution, and waterborne transmission of this pathogen. Here, we show that V. cholerae uses QS to regulate the expression of one locus required for V. cholerae-chitin interactions.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted July 03, 2020.
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Species-specific quorum sensing represses the chitobiose utilization locus in Vibrio cholerae
Catherine A. Klancher, Jane D. Newman, Alyssa S. Ball, Julia C. van Kessel, Ankur B. Dalia
bioRxiv 2020.04.16.045526; doi: https://doi.org/10.1101/2020.04.16.045526
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Species-specific quorum sensing represses the chitobiose utilization locus in Vibrio cholerae
Catherine A. Klancher, Jane D. Newman, Alyssa S. Ball, Julia C. van Kessel, Ankur B. Dalia
bioRxiv 2020.04.16.045526; doi: https://doi.org/10.1101/2020.04.16.045526

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