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Mass Spectrometric Identification of SARS-CoV-2 Proteins from Gargle Solution Samples of COVID-19 Patients

Christian Ihling, Dirk Tänzler, Sven Hagemann, Astrid Kehlen, Stefan Hüttelmaier, View ORCID ProfileAndrea Sinz
doi: https://doi.org/10.1101/2020.04.18.047878
Christian Ihling
1Department of Pharmaceutical Chemistry & Bioanalytics, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Charles Tanford Center, 06120 Halle, Germany
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Dirk Tänzler
1Department of Pharmaceutical Chemistry & Bioanalytics, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Charles Tanford Center, 06120 Halle, Germany
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Sven Hagemann
2Department of Molecular Cell Biology, Institute for Molecular Medicine, Charles Tanford Center, Medical Faculty, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany
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Astrid Kehlen
3Institute of Medicinal Microbiology, Biocenter, Medical Faculty, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany
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Stefan Hüttelmaier
2Department of Molecular Cell Biology, Institute for Molecular Medicine, Charles Tanford Center, Medical Faculty, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany
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Andrea Sinz
1Department of Pharmaceutical Chemistry & Bioanalytics, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Charles Tanford Center, 06120 Halle, Germany
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  • ORCID record for Andrea Sinz
  • For correspondence: andrea.sinz@pharmazie.uni-halle.de
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Abstract

Mass spectrometry (MS) can deliver valuable diagnostic data that complements genomic information and allows us to increase our current knowledge of the COVID-19 disease caused by the SARS-CoV-2 virus. We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. Our protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients.

Figure

Image credit (left): Gerd Altmann, Pixabay License, https://pixabay.com/illustrations/corona-coronavirus-virus-covid-19-4959447

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted April 19, 2020.
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Mass Spectrometric Identification of SARS-CoV-2 Proteins from Gargle Solution Samples of COVID-19 Patients
Christian Ihling, Dirk Tänzler, Sven Hagemann, Astrid Kehlen, Stefan Hüttelmaier, Andrea Sinz
bioRxiv 2020.04.18.047878; doi: https://doi.org/10.1101/2020.04.18.047878
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Mass Spectrometric Identification of SARS-CoV-2 Proteins from Gargle Solution Samples of COVID-19 Patients
Christian Ihling, Dirk Tänzler, Sven Hagemann, Astrid Kehlen, Stefan Hüttelmaier, Andrea Sinz
bioRxiv 2020.04.18.047878; doi: https://doi.org/10.1101/2020.04.18.047878

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