Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Genetic dissection of glutamatergic neuron subpopulations and developmental trajectories in the cerebral cortex

View ORCID ProfileKatherine S. Matho, View ORCID ProfileDhananjay Huilgol, View ORCID ProfileWilliam Galbavy, View ORCID ProfileMiao He, View ORCID ProfileGukhan Kim, View ORCID ProfileXu An, Jiangteng Lu, Priscilla Wu, View ORCID ProfileDaniela J. Di Bella, View ORCID ProfileAshwin S. Shetty, Ramesh Palaniswamy, Joshua Hatfield, Ricardo Raudales, View ORCID ProfileArun Narasimhan, Eric Gamache, Jesse Levine, Jason Tucciarone, Eric Szelenyi, View ORCID ProfileJulie A. Harris, View ORCID ProfilePartha P. Mitra, View ORCID ProfilePavel Osten, View ORCID ProfilePaola Arlotta, View ORCID ProfileZ. Josh Huang
doi: https://doi.org/10.1101/2020.04.22.054064
Katherine S. Matho
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Katherine S. Matho
Dhananjay Huilgol
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Dhananjay Huilgol
William Galbavy
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
4Program in Neuroscience, Department of Neurobiology and Behavior, Stony Brook University, Stony Brook, NY, 11794, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for William Galbavy
Miao He
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Miao He
Gukhan Kim
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Gukhan Kim
Xu An
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
7Department of Neurobiology, Duke University Medical Center, Durham, NC
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Xu An
Jiangteng Lu
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Priscilla Wu
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Daniela J. Di Bella
2Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Daniela J. Di Bella
Ashwin S. Shetty
2Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ashwin S. Shetty
Ramesh Palaniswamy
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joshua Hatfield
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
7Department of Neurobiology, Duke University Medical Center, Durham, NC
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ricardo Raudales
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
4Program in Neuroscience, Department of Neurobiology and Behavior, Stony Brook University, Stony Brook, NY, 11794, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Arun Narasimhan
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Arun Narasimhan
Eric Gamache
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jesse Levine
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
5Program in Neuroscience and Medical Scientist Training Program, Stony Brook University, New York 11790, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jason Tucciarone
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
5Program in Neuroscience and Medical Scientist Training Program, Stony Brook University, New York 11790, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eric Szelenyi
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julie A. Harris
6Allen Institute for Brain Science, Seattle, WA, 98109, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Julie A. Harris
Partha P. Mitra
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Partha P. Mitra
Pavel Osten
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Pavel Osten
Paola Arlotta
2Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
3Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02138, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Paola Arlotta
Z. Josh Huang
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
7Department of Neurobiology, Duke University Medical Center, Durham, NC
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Z. Josh Huang
  • For correspondence: josh.huang@duke.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

Diverse types of glutamatergic pyramidal neurons (PyNs) mediate the myriad processing streams and output channels of the cerebral cortex, yet all derive from neural progenitors of the embryonic dorsal telencephalon. Here, we establish genetic strategies and tools for dissecting and fate mapping PyN subpopulations based on their developmental and molecular programs. We leverage key transcription factors and effector genes to systematically target the temporal patterning programs in progenitors and differentiation programs in postmitotic neurons. We generated over a dozen temporally inducible mouse Cre and Flp knock-in driver lines to enable combinatorial targeting of major progenitor types and projection classes. Intersectional converter lines confer viral access to specific subsets defined by developmental origin, marker expression, anatomical location and projection targets. These strategies establish an experimental framework for understanding the hierarchical organization and developmental trajectory of PyN subpopulations that assemble cortical processing networks and output channels.

Competing Interest Statement

J.A.H. is currently employed by Cajal Neuroscience.

Footnotes

  • I am resubmitting due to a formatting issue with the previous version.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted March 16, 2021.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Genetic dissection of glutamatergic neuron subpopulations and developmental trajectories in the cerebral cortex
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Genetic dissection of glutamatergic neuron subpopulations and developmental trajectories in the cerebral cortex
Katherine S. Matho, Dhananjay Huilgol, William Galbavy, Miao He, Gukhan Kim, Xu An, Jiangteng Lu, Priscilla Wu, Daniela J. Di Bella, Ashwin S. Shetty, Ramesh Palaniswamy, Joshua Hatfield, Ricardo Raudales, Arun Narasimhan, Eric Gamache, Jesse Levine, Jason Tucciarone, Eric Szelenyi, Julie A. Harris, Partha P. Mitra, Pavel Osten, Paola Arlotta, Z. Josh Huang
bioRxiv 2020.04.22.054064; doi: https://doi.org/10.1101/2020.04.22.054064
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Genetic dissection of glutamatergic neuron subpopulations and developmental trajectories in the cerebral cortex
Katherine S. Matho, Dhananjay Huilgol, William Galbavy, Miao He, Gukhan Kim, Xu An, Jiangteng Lu, Priscilla Wu, Daniela J. Di Bella, Ashwin S. Shetty, Ramesh Palaniswamy, Joshua Hatfield, Ricardo Raudales, Arun Narasimhan, Eric Gamache, Jesse Levine, Jason Tucciarone, Eric Szelenyi, Julie A. Harris, Partha P. Mitra, Pavel Osten, Paola Arlotta, Z. Josh Huang
bioRxiv 2020.04.22.054064; doi: https://doi.org/10.1101/2020.04.22.054064

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (3579)
  • Biochemistry (7523)
  • Bioengineering (5486)
  • Bioinformatics (20699)
  • Biophysics (10260)
  • Cancer Biology (7939)
  • Cell Biology (11584)
  • Clinical Trials (138)
  • Developmental Biology (6573)
  • Ecology (10144)
  • Epidemiology (2065)
  • Evolutionary Biology (13552)
  • Genetics (9502)
  • Genomics (12794)
  • Immunology (7887)
  • Microbiology (19456)
  • Molecular Biology (7618)
  • Neuroscience (41916)
  • Paleontology (307)
  • Pathology (1253)
  • Pharmacology and Toxicology (2181)
  • Physiology (3253)
  • Plant Biology (7010)
  • Scientific Communication and Education (1291)
  • Synthetic Biology (1942)
  • Systems Biology (5410)
  • Zoology (1108)