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Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine induces equivalent preclinical antibody titers and viral neutralization to recovered COVID-19 patients

Paul F. McKay, Kai Hu, Anna K. Blakney, Karnyart Samnuan, Clément R. Bouton, Paul Rogers, Krunal Polra, Paulo J.C. Lin, Christopher Barbosa, Ying Tam, Robin J. Shattock
doi: https://doi.org/10.1101/2020.04.22.055608
Paul F. McKay
1Department of Infectious Diseases, Imperial College London, Norfolk Place, London, United Kingdom W2 1PG
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Kai Hu
1Department of Infectious Diseases, Imperial College London, Norfolk Place, London, United Kingdom W2 1PG
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Anna K. Blakney
1Department of Infectious Diseases, Imperial College London, Norfolk Place, London, United Kingdom W2 1PG
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Karnyart Samnuan
1Department of Infectious Diseases, Imperial College London, Norfolk Place, London, United Kingdom W2 1PG
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Clément R. Bouton
1Department of Infectious Diseases, Imperial College London, Norfolk Place, London, United Kingdom W2 1PG
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Paul Rogers
1Department of Infectious Diseases, Imperial College London, Norfolk Place, London, United Kingdom W2 1PG
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Krunal Polra
1Department of Infectious Diseases, Imperial College London, Norfolk Place, London, United Kingdom W2 1PG
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Paulo J.C. Lin
2Acuitas Therapeutics, Vancouver, British Columbia, Canada, V6T 1Z3
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Christopher Barbosa
2Acuitas Therapeutics, Vancouver, British Columbia, Canada, V6T 1Z3
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Ying Tam
2Acuitas Therapeutics, Vancouver, British Columbia, Canada, V6T 1Z3
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Robin J. Shattock
1Department of Infectious Diseases, Imperial College London, Norfolk Place, London, United Kingdom W2 1PG
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  • For correspondence: r.shattock@imperial.ac.uk
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ABSTRACT

The spread of the SARS-CoV-2 into a global pandemic within a few months of onset motivates the development of a rapidly scalable vaccine. Here, we present a self-amplifying RNA encoding the SARS-CoV-2 spike protein encapsulated within a lipid nanoparticle as a vaccine and demonstrate induction of robust neutralization of a pseudo-virus, proportional to quantity of specific IgG and of higher quantities than recovered COVID-19 patients. These data provide insight into the vaccine design and evaluation of immunogenicity to enable rapid translation to the clinic.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted April 25, 2020.
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Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine induces equivalent preclinical antibody titers and viral neutralization to recovered COVID-19 patients
Paul F. McKay, Kai Hu, Anna K. Blakney, Karnyart Samnuan, Clément R. Bouton, Paul Rogers, Krunal Polra, Paulo J.C. Lin, Christopher Barbosa, Ying Tam, Robin J. Shattock
bioRxiv 2020.04.22.055608; doi: https://doi.org/10.1101/2020.04.22.055608
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Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine induces equivalent preclinical antibody titers and viral neutralization to recovered COVID-19 patients
Paul F. McKay, Kai Hu, Anna K. Blakney, Karnyart Samnuan, Clément R. Bouton, Paul Rogers, Krunal Polra, Paulo J.C. Lin, Christopher Barbosa, Ying Tam, Robin J. Shattock
bioRxiv 2020.04.22.055608; doi: https://doi.org/10.1101/2020.04.22.055608

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