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Diversity and gene expression patterns of functional groups in sidestream and mainstream wastewater partial-nitritation anammox biofilms

View ORCID ProfileCarolina Suarez, David Gustavsson, Malte Hermansson, Frank Persson
doi: https://doi.org/10.1101/2020.04.23.057489
Carolina Suarez
1Department of Chemistry and Molecular Biology, University of Gothenburg, SE-40530 Gothenburg, Sweden
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  • For correspondence: carolina.suarez@cmb.gu.se
David Gustavsson
2VA SYD, P.O. Box 191, SE-20121 Malmö, Sweden
3Sweden Water Research, c/o Ideon Science Park, Scheelevägen 15, SE-22370 Lund, Sweden
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Malte Hermansson
1Department of Chemistry and Molecular Biology, University of Gothenburg, SE-40530 Gothenburg, Sweden
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Frank Persson
4Division of Water Environment Technology, Department of Architecture and Civil Engineering, Chalmers University of Technology, SE-41296 Gothenburg, Sweden
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Abstract

Partial nitritation-anammox (PNA) is today used for nitrogen removal from highly concentrated wastewater after anaerobic sludge digestion (sidestream). However, implementation of PNA for treatment of municipal wastewater (mainstream), with its lower ammonium concentration and lower temperature is challenging, which might be due to differences in microbial community composition and/or activity. To investigate this, we compared side-by-side sidestream and mainstream PNA biofilms using amplicon sequencing of 16S rDNA and rRNA, hzsB DNA and mRNA, and the genes nxrB, and amoA. The two communities were different to each other with relatively more heterotrophic denitrifying bacteria and less anammox bacteria in the mainstream. With hzsB and nxrB we found microdiversity among Brocadia and Nitrospira, and turnover (taxa replacement) between sidestream and mainstream. However, in both environments Brocadia sapporoensis represented most of the hzsB DNA and mRNA reads, despite the different environmental conditions and nitrogen removal rates. All of those populations present in both sidestream and mainstream had no differences in their 16S rRNA:rDNA ratios, supporting recent findings that rRNA:rDNA ratios are poor indicators of bacterial activity. The observed diversity within functional groups and composition differences between sidestream and mainstream add complexity to our view of PNA communities with possible implication for reactor function.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted April 25, 2020.
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Diversity and gene expression patterns of functional groups in sidestream and mainstream wastewater partial-nitritation anammox biofilms
Carolina Suarez, David Gustavsson, Malte Hermansson, Frank Persson
bioRxiv 2020.04.23.057489; doi: https://doi.org/10.1101/2020.04.23.057489
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Diversity and gene expression patterns of functional groups in sidestream and mainstream wastewater partial-nitritation anammox biofilms
Carolina Suarez, David Gustavsson, Malte Hermansson, Frank Persson
bioRxiv 2020.04.23.057489; doi: https://doi.org/10.1101/2020.04.23.057489

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