ABSTRACT
In this article, the principle of a database aimed at facilitating the understanding of the unique protein nucleating properties of the Crystallophore is presented. A first analysis allows us to compare the efficiency of Tb-Xo4 with the new Lu-Xo4 variant, featuring improved phasing properties. Then, the concept of subset-of-interest is introduced to reveal potential antagonistic/synergistic effects between Tb-Xo4 and physico-chemical parameters of the crystallisation kits such as pH. The overall approach may be of interest for any studies working on solutions dedicated to improve the nucleating step in protein crystallization.
Synopsis A database and associated representation tools are highlighted to understand nucleating properties of the crystallophore.
Competing Interest Statement
OM, FR, EG and SE declare a potential conflict of interest since they are co-founders of the Polyvalan company that commercializes the crystallophore.
ABBREVIATIONS
- Xo4
- crystallophore;
- SOI
- subset-of-interest;
- HEWL
- Hen egg white lysozyme;
- GRHPR
- Glyoxylate Hydroxypyruvate Reductase.
