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Tracking crystallophore nucleating properties: setting-up a database for statistical analysis

Tao Jiang, View ORCID ProfileAmandine Roux, View ORCID ProfileSylvain Engilberge, Zaynab Alsalman, View ORCID ProfileSebastiano Di Pietro, Bruno Franzetti, View ORCID ProfileFrançois Riobé, Olivier Maury, View ORCID ProfileEric Girard
doi: https://doi.org/10.1101/2020.04.23.057596
Tao Jiang
1Univ. Lyon, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard Lyon 1, Laboratoire de Chimie, F69342, Lyon, France
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Amandine Roux
1Univ. Lyon, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard Lyon 1, Laboratoire de Chimie, F69342, Lyon, France
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  • ORCID record for Amandine Roux
Sylvain Engilberge
2Univ. Grenoble Alpes, CEA, CNRS, Institut de Biologie Structurale, 38000 Grenoble, France
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  • ORCID record for Sylvain Engilberge
Zaynab Alsalman
2Univ. Grenoble Alpes, CEA, CNRS, Institut de Biologie Structurale, 38000 Grenoble, France
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Sebastiano Di Pietro
1Univ. Lyon, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard Lyon 1, Laboratoire de Chimie, F69342, Lyon, France
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Bruno Franzetti
2Univ. Grenoble Alpes, CEA, CNRS, Institut de Biologie Structurale, 38000 Grenoble, France
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François Riobé
1Univ. Lyon, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard Lyon 1, Laboratoire de Chimie, F69342, Lyon, France
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  • For correspondence: eric.girard@ibs.fr olivier.maury@ens-lyon.fr francois.riobe@ens-lyon.fr
Olivier Maury
1Univ. Lyon, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard Lyon 1, Laboratoire de Chimie, F69342, Lyon, France
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  • For correspondence: eric.girard@ibs.fr olivier.maury@ens-lyon.fr francois.riobe@ens-lyon.fr
Eric Girard
2Univ. Grenoble Alpes, CEA, CNRS, Institut de Biologie Structurale, 38000 Grenoble, France
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  • For correspondence: eric.girard@ibs.fr olivier.maury@ens-lyon.fr francois.riobe@ens-lyon.fr
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ABSTRACT

In this article, the principle of a database aimed at facilitating the understanding of the unique protein nucleating properties of the Crystallophore is presented. A first analysis allows us to compare the efficiency of Tb-Xo4 with the new Lu-Xo4 variant, featuring improved phasing properties. Then, the concept of subset-of-interest is introduced to reveal potential antagonistic/synergistic effects between Tb-Xo4 and physico-chemical parameters of the crystallisation kits such as pH. The overall approach may be of interest for any studies working on solutions dedicated to improve the nucleating step in protein crystallization.

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Synopsis A database and associated representation tools are highlighted to understand nucleating properties of the crystallophore.

Competing Interest Statement

OM, FR, EG and SE declare a potential conflict of interest since they are co-founders of the Polyvalan company that commercializes the crystallophore.

  • ABBREVIATIONS

    Xo4
    crystallophore;
    SOI
    subset-of-interest;
    HEWL
    Hen egg white lysozyme;
    GRHPR
    Glyoxylate Hydroxypyruvate Reductase.
  • Copyright 
    The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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    Posted April 25, 2020.
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    Tracking crystallophore nucleating properties: setting-up a database for statistical analysis
    Tao Jiang, Amandine Roux, Sylvain Engilberge, Zaynab Alsalman, Sebastiano Di Pietro, Bruno Franzetti, François Riobé, Olivier Maury, Eric Girard
    bioRxiv 2020.04.23.057596; doi: https://doi.org/10.1101/2020.04.23.057596
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    Tracking crystallophore nucleating properties: setting-up a database for statistical analysis
    Tao Jiang, Amandine Roux, Sylvain Engilberge, Zaynab Alsalman, Sebastiano Di Pietro, Bruno Franzetti, François Riobé, Olivier Maury, Eric Girard
    bioRxiv 2020.04.23.057596; doi: https://doi.org/10.1101/2020.04.23.057596

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