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Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens

Karel Bezstarosti, View ORCID ProfileMart M. Lamers, Jeroen J. A. van Kampen, View ORCID ProfileBart L. Haagmans, View ORCID ProfileJeroen A. A. Demmers
doi: https://doi.org/10.1101/2020.04.23.057810
Karel Bezstarosti
1Proteomics Center, Erasmus University Medical Center, Rotterdam, the Netherlands
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Mart M. Lamers
2Viroscience Department, Erasmus University Medical Center, Rotterdam, the Netherlands
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Jeroen J. A. van Kampen
2Viroscience Department, Erasmus University Medical Center, Rotterdam, the Netherlands
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Bart L. Haagmans
2Viroscience Department, Erasmus University Medical Center, Rotterdam, the Netherlands
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Jeroen A. A. Demmers
1Proteomics Center, Erasmus University Medical Center, Rotterdam, the Netherlands
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  • For correspondence: j.demmers@erasmusmc.nl
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ABSTRACT

The rapid, sensitive and specific detection of SARS-CoV-2 is critical in responding to the current COVID-19 outbreak. Here, we explore the potential of targeted mass spectrometry based proteomics for the detection of SARS-CoV-2 proteins in both research and clinical samples. First, we assessed the limit of detection for several SARS-CoV-2 proteins by parallel reaction monitoring (PRM) mass spectrometry. For Nucleocapsid the limit of detection was found to be in the mid-attomole range (0.9 x 10−12 g). Next, we apply this PRM assay to the detection of viral proteins in in vitro mucus substitutes, as well as in various clinical specimens such as nasopharyngeal swabs and sputum. In this proof-of-concept study SARS-CoV-2 proteins could unambiguously be detected in clinical samples, suggesting that the sensitivity of this technology may be sufficiently high to further explore its potential role in diagnostics.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Manuscript now includes data from COVID-19 patient samples.

  • https://www.ebi.ac.uk/pride/

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted December 18, 2020.
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Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens
Karel Bezstarosti, Mart M. Lamers, Jeroen J. A. van Kampen, Bart L. Haagmans, Jeroen A. A. Demmers
bioRxiv 2020.04.23.057810; doi: https://doi.org/10.1101/2020.04.23.057810
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Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens
Karel Bezstarosti, Mart M. Lamers, Jeroen J. A. van Kampen, Bart L. Haagmans, Jeroen A. A. Demmers
bioRxiv 2020.04.23.057810; doi: https://doi.org/10.1101/2020.04.23.057810

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