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Fluorogenic probe for fast 3D whole-cell DNA-PAINT

View ORCID ProfileKenny KH Chung, Zhao Zhang, View ORCID ProfilePhylicia Kidd, View ORCID ProfileYongdeng Zhang, View ORCID ProfileNathan D Williams, Bennett Rollins, View ORCID ProfileYang Yang, View ORCID ProfileChenxiang Lin, View ORCID ProfileDavid Baddeley, View ORCID ProfileJoerg Bewersdorf
doi: https://doi.org/10.1101/2020.04.29.066886
Kenny KH Chung
1Department of Cell Biology, Yale University
2Nanobiology Institute, Yale University
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Zhao Zhang
1Department of Cell Biology, Yale University
2Nanobiology Institute, Yale University
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Phylicia Kidd
1Department of Cell Biology, Yale University
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Yongdeng Zhang
1Department of Cell Biology, Yale University
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Nathan D Williams
1Department of Cell Biology, Yale University
2Nanobiology Institute, Yale University
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Bennett Rollins
1Department of Cell Biology, Yale University
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Yang Yang
1Department of Cell Biology, Yale University
2Nanobiology Institute, Yale University
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Chenxiang Lin
1Department of Cell Biology, Yale University
2Nanobiology Institute, Yale University
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David Baddeley
1Department of Cell Biology, Yale University
2Nanobiology Institute, Yale University
3Auckland Bioengineering Institute, University of Auckland
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Joerg Bewersdorf
1Department of Cell Biology, Yale University
2Nanobiology Institute, Yale University
4Department of Biomedical Engineering, Yale University
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  • ORCID record for Joerg Bewersdorf
  • For correspondence: joerg.bewersdorf@yale.edu
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Abstract

DNA-PAINT is an increasingly popular super-resolution microscopy method that can acquire high-fidelity images at nanometer resolution. It suffers, however, from high background and very slow imaging speed, both of which can be attributed to the presence of unbound fluorophores in solution. We present a fluorogenic DNA-PAINT probe that solves these problems and demonstrate 3D imaging without the need for optical sectioning and a 26-fold increase in imaging speed over regular DNA-PAINT.

Competing Interest Statement

J. B. discloses significant financial interest in Bruker Corp. and Hamamatsu Photonics.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 30, 2020.
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Fluorogenic probe for fast 3D whole-cell DNA-PAINT
Kenny KH Chung, Zhao Zhang, Phylicia Kidd, Yongdeng Zhang, Nathan D Williams, Bennett Rollins, Yang Yang, Chenxiang Lin, David Baddeley, Joerg Bewersdorf
bioRxiv 2020.04.29.066886; doi: https://doi.org/10.1101/2020.04.29.066886
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Fluorogenic probe for fast 3D whole-cell DNA-PAINT
Kenny KH Chung, Zhao Zhang, Phylicia Kidd, Yongdeng Zhang, Nathan D Williams, Bennett Rollins, Yang Yang, Chenxiang Lin, David Baddeley, Joerg Bewersdorf
bioRxiv 2020.04.29.066886; doi: https://doi.org/10.1101/2020.04.29.066886

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