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The Spatial Scale of Synaptic Protein Allocation during Homeostatic Plasticity

View ORCID ProfileChao Sun, Andreas Nold, Tatjana Tchumatchenko, Mike Heilemann, Erin M. Schuman
doi: https://doi.org/10.1101/2020.04.29.068833
Chao Sun
1Max Planck Institute for Brain Research, Frankfurt, Germany
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Andreas Nold
1Max Planck Institute for Brain Research, Frankfurt, Germany
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Tatjana Tchumatchenko
1Max Planck Institute for Brain Research, Frankfurt, Germany
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Mike Heilemann
2Institute of Physical and Theoretical Chemistry, Goethe University, Frankfurt, Germany
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Erin M. Schuman
1Max Planck Institute for Brain Research, Frankfurt, Germany
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  • For correspondence: erin.schuman@brain.mpg.de
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Abstract

An individual neuron hosts up to 10,000 individual synapses that can be made stronger or weaker by local and cell-wide plasticity mechanisms, both of which require protein synthesis. To address over what spatial scale a neuron allocates synaptic resources, we quantified the distribution of newly synthesized proteins after global homeostatic upscaling using metabolic labeling and single-molecule localization (DNA-PAINT). Following upscaling, we observed a global increase in locally synthesized nascent protein in synapses and at dendrites, with a high degree of variability between individual synapses. We determined the smallest spatial scale over which nascent proteins were evenly distributed and found that it is best described by synaptic neighborhoods (~ 10 microns in length)-smaller than a dendritic branch and larger than an individual synapse. Protein allocation at the level of neighborhoods thus represents a solution to the problem of protein allocation within a neuron that balances local autonomy and global homeostasis.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted April 30, 2020.
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The Spatial Scale of Synaptic Protein Allocation during Homeostatic Plasticity
Chao Sun, Andreas Nold, Tatjana Tchumatchenko, Mike Heilemann, Erin M. Schuman
bioRxiv 2020.04.29.068833; doi: https://doi.org/10.1101/2020.04.29.068833
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The Spatial Scale of Synaptic Protein Allocation during Homeostatic Plasticity
Chao Sun, Andreas Nold, Tatjana Tchumatchenko, Mike Heilemann, Erin M. Schuman
bioRxiv 2020.04.29.068833; doi: https://doi.org/10.1101/2020.04.29.068833

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