Automatic deep learning driven label-free image guided patch clamp system for human and rodent in vitro slice physiology

Krisztian Koos; Gáspár Oláh; Tamas Balassa; Norbert Mihut; Márton Rózsa; Attila Ozsvár; Ervin Tasnadi; Pál Barzó; Nóra Faragó; László Puskás; Gábor Molnár; József Molnár; Gábor Tamás; Peter Horvath

doi:10.1101/2020.05.05.078162

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Automatic deep learning driven label-free image guided patch clamp system for human and rodent in vitro slice physiology

Krisztian Koos, Gáspár Oláh, Tamas Balassa, Norbert Mihut, Márton Rózsa, Attila Ozsvár, Ervin Tasnadi, Pál Barzó, Nóra Faragó, László Puskás, Gábor Molnár, József Molnár, Gábor Tamás, Peter Horvath

doi: https://doi.org/10.1101/2020.05.05.078162

Krisztian Koos

1Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary

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Gáspár Oláh

2MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Hungary

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Tamas Balassa

1Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary

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Norbert Mihut

2MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Hungary

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Márton Rózsa

2MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Hungary

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Attila Ozsvár

2MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Hungary

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Ervin Tasnadi

1Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary

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Pál Barzó

3Department of Neurosurgery, University of Szeged, Szeged, Hungary

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Nóra Faragó

2MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Hungary

4Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre, Szeged, Hungary

5Avidin Ltd, Szeged, Hungary

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László Puskás

4Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre, Szeged, Hungary

5Avidin Ltd, Szeged, Hungary

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Gábor Molnár

2MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Hungary

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József Molnár

1Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary

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Gábor Tamás

2MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Hungary

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Peter Horvath

1Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary

6Institute for Molecular Medicine Finland, University of Helsinki, Helsinki, Finland

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For correspondence: horvath.peter@brc.hu

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Supplementary Information: Cell Detection System[supplements/078162_file02.pdf]
Supplementary Information: Cell Tracking System[supplements/078162_file03.pdf]
Supplementary Information: Pipette Detection System[supplements/078162_file04.pdf]
Supplementary Information: Pressure Regulator[supplements/078162_file05.pdf]
Supplementary Information: Software Usage[supplements/078162_file06.pdf]