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A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues

View ORCID ProfileAdam K. Glaser, Kevin W. Bishop, Lindsey A. Barner, View ORCID ProfileRobert B. Serafin, View ORCID ProfileJonathan T.C. Liu
doi: https://doi.org/10.1101/2020.05.06.081745
Adam K. Glaser
1Department of Mechanical Engineering, University of Washington, Seattle, WA USA
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  • For correspondence: akglaser@uw.edu jonliu@uw.edu
Kevin W. Bishop
1Department of Mechanical Engineering, University of Washington, Seattle, WA USA
2Department of Bioengineering, University of Washington, Seattle, WA USA
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Lindsey A. Barner
1Department of Mechanical Engineering, University of Washington, Seattle, WA USA
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Robert B. Serafin
1Department of Mechanical Engineering, University of Washington, Seattle, WA USA
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Jonathan T.C. Liu
1Department of Mechanical Engineering, University of Washington, Seattle, WA USA
2Department of Bioengineering, University of Washington, Seattle, WA USA
3Department of Pathology, University of Washington, Seattle, WA USA
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  • For correspondence: akglaser@uw.edu jonliu@uw.edu
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Abstract

Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need for a user-friendly system that can address diverse imaging applications with varied requirements in terms of resolution (mesoscopic to sub-micron), sample geometry (size, shape, and number), and compatibility with tissue-clearing protocols of different refractive indices. We present a hybrid system that combines a novel non-orthogonal dual-objective and conventional open-top light-sheet architecture for highly versatile multi-scale volumetric imaging.

One sentence summary Glaser et al. describe a hybrid open-top light-sheet microscope to image cleared tissues at mesoscopic to sub-micron resolution and depths of up to 1 cm.

Competing Interest Statement

A.K.G. and J.T.C.L. are co-founders and shareholders of Lightspeed Microscopy Inc.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted May 08, 2020.
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A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues
Adam K. Glaser, Kevin W. Bishop, Lindsey A. Barner, Robert B. Serafin, Jonathan T.C. Liu
bioRxiv 2020.05.06.081745; doi: https://doi.org/10.1101/2020.05.06.081745
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A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues
Adam K. Glaser, Kevin W. Bishop, Lindsey A. Barner, Robert B. Serafin, Jonathan T.C. Liu
bioRxiv 2020.05.06.081745; doi: https://doi.org/10.1101/2020.05.06.081745

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