Abstract
Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need for a user-friendly system that can address imaging applications with varied requirements in terms of resolution (mesoscopic to sub-micrometer), sample geometry (size, shape, and number), and compatibility with tissue-clearing protocols and sample holders of various refractive indices. We present a ‘hybrid’ system that combines a novel non-orthogonal dual-objective and conventional (orthogonal) open-top light-sheet architecture for versatile multi-scale volumetric imaging.
Competing Interest Statement
A.K.G., N.P.R., L.D.T., and J.T.C.L. are co-founders and shareholders of Lightspeed Microscopy Inc.
Footnotes
The manuscript now has experimental data which was not present in the original manuscript.
