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An Optimal Lysis Time Maximizes Bacteriophage Fitness in Quasi-continuous Culture

Sherin Kannoly, Abhyudai Singh, View ORCID ProfileJohn J. Dennehy
doi: https://doi.org/10.1101/2020.05.11.089508
Sherin Kannoly
1Biology Department, Queens College of the City University of New York, NYC, NY, USA
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Abhyudai Singh
2Department of Electrical and Computer Engineering, University of Delaware, Newark, DE, USA
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  • For correspondence: absingh@udel.edu john.dennehy@qc.cuny.edu
John J. Dennehy
1Biology Department, Queens College of the City University of New York, NYC, NY, USA
3The Graduate Center of the City University of New York, NYC, NY, USA
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  • ORCID record for John J. Dennehy
  • For correspondence: absingh@udel.edu john.dennehy@qc.cuny.edu
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ABSTRACT

Optimality models have a checkered history in evolutionary biology. While optimality models have been successful in providing valuable insight into the evolution of a wide variety of biological traits, a common objection is that optimality models are overly simplistic and ignore organismal genetics. We revisit evolutionary optimization in the context of a major bacteriophage life history trait, lysis time. Lysis time refers to the period spanning phage infection of a host cell and its lysis, whereupon phage progeny are released. Lysis time, therefore, directly determines phage fecundity assuming progeny assembly rate is maximized. Noting that previous tests of lysis time optimality rely on batch culture, we implemented a quasi-steady state system to observe productivity of a panel of isogenic phage λ mutants differing in lysis time. We report that λ phage productivity in our experiments is maximized around an optimal lysis time of 65 min, which is the lysis time of the λ “wildtype” strain. We discuss this finding in light of recent results that lysis time variation is also minimized in the λ “wildtype” strain.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted May 12, 2020.
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An Optimal Lysis Time Maximizes Bacteriophage Fitness in Quasi-continuous Culture
Sherin Kannoly, Abhyudai Singh, John J. Dennehy
bioRxiv 2020.05.11.089508; doi: https://doi.org/10.1101/2020.05.11.089508
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An Optimal Lysis Time Maximizes Bacteriophage Fitness in Quasi-continuous Culture
Sherin Kannoly, Abhyudai Singh, John J. Dennehy
bioRxiv 2020.05.11.089508; doi: https://doi.org/10.1101/2020.05.11.089508

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