Abstract
Here we report the first method for rapid on-site monitoring of germfree C57BL/6 mice colonized with feces from conventionally raised mice. The method, named levô in fimo test, readily distinguishes feces from germfree mice and conventionalized germfree mice based on its properties to float or sink in Trump’s fixative solution. We validated the test using feces from conventionally raised mice, germfree mice and conventionalized germfree mice, and cross-validated using multiple indicators of microbial presence in feces. Levô in fimo test is a simple, cost-effective, instant yet robust method for on-site monitoring of gut microbial reconstitution in germfree mice.
Competing Interest Statement
The authors have declared no competing interest.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Posted May 12, 2020.
