Article Information
History
- May 13, 2020.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Author Information
- Basil J. Grebera,b,1,
- Juan M. Perez-Bertoldic,
- Kif Limd,
- Anthony T. Iavaronee,
- Daniel B. Tosoa and
- Eva Nogalesa,b,d,f,1
- aCalifornia Institute for Quantitative Biosciences (QB3), University of California, Berkeley, California 94720, USA
- bMolecular Biophysics and Integrative Bio-Imaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
- cBiophysics Graduate Group, University of California, Berkeley, California 94720, USA
- dDepartment of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
- eQB3/Chemistry Mass Spectrometry Facility, University of California, Berkeley, California 94720, USA
- fHoward Hughes Medical Institute, University of California, Berkeley, California 94720, USA
- ↵1To whom correspondence may be addressed: Eva Nogales, Basil J. Greber, Email: ENogales{at}lbl.gov, basilgreber{at}berkeley.edu
Author Contributions B.J.G. and K.L. cloned CAK expression constructs and purified wild-type CAK. J.M.P.B and B.J.G. performed activity assays, prepared the CAK-THZ1 complex and determined its initial structure. A.T.I performed mass spectrometric analyses. B.J.G. and D.B.T collected the cryo-EM data. B.J.G. processed the cryo-EM data, built and refined the coordinate models, and wrote the initial draft of the manuscript. All authors contributed to the final version of the paper.
Posted May 13, 2020.
