Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

A system-view of B. pertussis booster vaccine responses in adults primed with whole-cell vs. acellular vaccine in infancy

View ORCID ProfileRicardo da Silva Antunes, Mikhail Pomaznoy, View ORCID ProfileFerran Soldevila, Mariana Babor, Jason Bennett, Yuan Tian, Natalie Khalil, Yu Qian, Aishwarya Mandava, View ORCID ProfileRichard H. Scheuermann, Mario Cortese, View ORCID ProfileBali Pulendran, Christopher D. Petro, Adrienne Gilkes, Lisa A. Purcell, View ORCID ProfileAlessandro Sette, View ORCID ProfileBjoern Peters
doi: https://doi.org/10.1101/2020.05.15.098830
Ricardo da Silva Antunes
1Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ricardo da Silva Antunes
Mikhail Pomaznoy
1Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ferran Soldevila
1Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ferran Soldevila
Mariana Babor
1Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jason Bennett
1Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yuan Tian
1Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Natalie Khalil
1Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yu Qian
2J. Craig Venter Institute, La Jolla, California, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aishwarya Mandava
2J. Craig Venter Institute, La Jolla, California, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Richard H. Scheuermann
2J. Craig Venter Institute, La Jolla, California, United States
5University of California San Diego School of Medicine, La Jolla, California, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Richard H. Scheuermann
Mario Cortese
3Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bali Pulendran
3Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Bali Pulendran
Christopher D. Petro
4Regeneron Pharmaceuticals, Inc., Tarrytown, New York, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Adrienne Gilkes
4Regeneron Pharmaceuticals, Inc., Tarrytown, New York, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lisa A. Purcell
4Regeneron Pharmaceuticals, Inc., Tarrytown, New York, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alessandro Sette
1Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, United States
5University of California San Diego School of Medicine, La Jolla, California, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Alessandro Sette
Bjoern Peters
1Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, United States
5University of California San Diego School of Medicine, La Jolla, California, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Bjoern Peters
  • For correspondence: bpeters@lji.org
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Whole-cell inactivated vaccine against Bordetella pertussis (wP) was substituted in many countries by an acellular subunit vaccine (aP) to reduce side effects. Recent epidemiological studies have shown that aP vaccination in infancy induces less durable immunity than wP vaccination. To determine immunological differences associated with aP vs. wP priming, we performed system-level profiling of the immune response in adults primed with aP vs. wP vaccine in infancy following the Tdap booster vaccination as a surrogate to antigen encounter in vivo. Shared immune responses across cohorts were identified, including an increase of the blood monocyte frequency on day 1, and strong antigen-specific IgG response seven days after boost. Comparing aP and wP primed individuals, we found a subset of aP-primed individuals with higher levels of expression for several genes including CCL3 on day 3 and NFKBIA and ICAM1 on day 7 post immunization. These observations were supported by increased CCL3 concentrations in plasma of aP primed individuals. Contrary to the wP individuals, the CCL3-high aP subset presented boosted PT-specific IgE responses. Furthermore, higher antigen specific IgG4 and IgG3 antibodies against specific vaccine antigens at baseline and post boost of aP individuals was observed, suggesting a long term maintained difference in the IgG subtype response. Overall our findings demonstrate that, while broad immune response patterns to Tdap boost overlap between aP and wP primed individuals, a subset of aP primed individuals present a divergent response. These findings provide candidate targets to study the causes and correlates of waning immunity after aP vaccination.

Competing Interest Statement

A.G., C.D.P. and L.A.P. are employees and shareholders of Regeneron Pharmaceuticals, Inc.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted May 16, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
A system-view of B. pertussis booster vaccine responses in adults primed with whole-cell vs. acellular vaccine in infancy
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
A system-view of B. pertussis booster vaccine responses in adults primed with whole-cell vs. acellular vaccine in infancy
Ricardo da Silva Antunes, Mikhail Pomaznoy, Ferran Soldevila, Mariana Babor, Jason Bennett, Yuan Tian, Natalie Khalil, Yu Qian, Aishwarya Mandava, Richard H. Scheuermann, Mario Cortese, Bali Pulendran, Christopher D. Petro, Adrienne Gilkes, Lisa A. Purcell, Alessandro Sette, Bjoern Peters
bioRxiv 2020.05.15.098830; doi: https://doi.org/10.1101/2020.05.15.098830
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
A system-view of B. pertussis booster vaccine responses in adults primed with whole-cell vs. acellular vaccine in infancy
Ricardo da Silva Antunes, Mikhail Pomaznoy, Ferran Soldevila, Mariana Babor, Jason Bennett, Yuan Tian, Natalie Khalil, Yu Qian, Aishwarya Mandava, Richard H. Scheuermann, Mario Cortese, Bali Pulendran, Christopher D. Petro, Adrienne Gilkes, Lisa A. Purcell, Alessandro Sette, Bjoern Peters
bioRxiv 2020.05.15.098830; doi: https://doi.org/10.1101/2020.05.15.098830

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Systems Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4655)
  • Biochemistry (10308)
  • Bioengineering (7629)
  • Bioinformatics (26208)
  • Biophysics (13454)
  • Cancer Biology (10631)
  • Cell Biology (15354)
  • Clinical Trials (138)
  • Developmental Biology (8458)
  • Ecology (12761)
  • Epidemiology (2067)
  • Evolutionary Biology (16777)
  • Genetics (11361)
  • Genomics (15411)
  • Immunology (10557)
  • Microbiology (25063)
  • Molecular Biology (10163)
  • Neuroscience (54131)
  • Paleontology (398)
  • Pathology (1656)
  • Pharmacology and Toxicology (2878)
  • Physiology (4318)
  • Plant Biology (9206)
  • Scientific Communication and Education (1582)
  • Synthetic Biology (2543)
  • Systems Biology (6757)
  • Zoology (1453)