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Architecture and self-assembly of the SARS-CoV-2 nucleocapsid protein

View ORCID ProfileQiaozhen Ye, Alan M.V. West, Steve Silletti, View ORCID ProfileKevin D. Corbett
doi: https://doi.org/10.1101/2020.05.17.100685
Qiaozhen Ye
1Department of Cellular & Molecular Medicine, University of California San Diego, La Jolla, CA
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Alan M.V. West
1Department of Cellular & Molecular Medicine, University of California San Diego, La Jolla, CA
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Steve Silletti
2Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA
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Kevin D. Corbett
1Department of Cellular & Molecular Medicine, University of California San Diego, La Jolla, CA
2Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA
3Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, CA
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  • For correspondence: kcorbett@ucsd.edu
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Abstract

The COVID-2019 pandemic is the most severe acute public health threat of the twenty-first century. To properly address this crisis with both robust testing and novel treatments, we require a deep understanding of the life cycle of the causative agent, the SARS-CoV-2 coronavirus. Here, we examine the architecture and self-assembly properties of the SARS-CoV-2 nucleocapsid (N) protein, which binds viral RNA and assembles into a filament that is packaged into new virions. We determined a 1.4 Å resolution crystal structure of this protein’s N2b domain, revealing a compact, intertwined dimer very similar to that of related coronaviruses SARS-CoV and MERS-CoV. Using size exclusion chromatography and multi-angle light scattering, we find that this domain forms a dimer in solution, and that addition of the C-terminal spacer B/N3 domain mediates tetramer formation. Using hydrogen-deuterium exchange mass spectrometry, we find evidence that at least part of this putatively disordered domain is structured, potentially forming an α-helix that either self-associates or docks against the N2b domain to mediate tetramer formation. Finally, we map the locations of over 4,400 individual amino acid substitutions in the N protein from ~17,000 SARS-CoV-2 genome sequences, and find that they are strongly clustered in the protein’s N2a linker domain. The nearly 300 substitutions identified within the N1b and N2b domains cluster away from their functional RNA binding and dimerization interfaces. Overall, this work reveals the architecture and self-assembly properties of a key protein in the SARS-CoV-2 life cycle. As the N protein is a common target of patient antibodies, this work will also benefit ongoing efforts to develop robust and specific serological tests, and could also benefit the analysis of patient-derived antibodies.

Significance Statement After infecting a cell, the SARS-CoV-2 coronavirus replicates its genome and packages it into new virus particles. The virus’s nucleocapsid (N) protein is critical for this process, binding the genomic RNA and assembling into large filaments before being packaged into new virions. Here, we sought to understand the structure of the SARS-CoV-2 N protein and how it forms large assemblies. We combined x-ray crystallography and two biochemical methods to show that the protein’s N2b and N3 domains mediate self-association into dimers and tetramers, respectively. We have therefore outlined the first two steps of a probable three-step self-assembly mechanism of the SARS-CoV-2 nucleocapsid. This work will benefit ongoing efforts to develop robust viral tests and to develop new drugs targeting key steps in the viral life cycle.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 17, 2020.
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Architecture and self-assembly of the SARS-CoV-2 nucleocapsid protein
Qiaozhen Ye, Alan M.V. West, Steve Silletti, Kevin D. Corbett
bioRxiv 2020.05.17.100685; doi: https://doi.org/10.1101/2020.05.17.100685
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Architecture and self-assembly of the SARS-CoV-2 nucleocapsid protein
Qiaozhen Ye, Alan M.V. West, Steve Silletti, Kevin D. Corbett
bioRxiv 2020.05.17.100685; doi: https://doi.org/10.1101/2020.05.17.100685

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