New Results
Massively parallel quantification of CRISPR editing in cells by TRAP-seq enables better design of Cas9, ABE, CBE gRNAs of high efficiency and accuracy
Xi Xiang, Kunli Qu, Xue Liang, Xiaoguang Pan, Jun Wang, Peng Han, Zhanying Dong, Lijun Liu, Jiayan Zhong, Tao Ma, Yiqing Wang, Jiaying Yu, Xiaoying Zhao, Siyuan Li, Zhe Xu, Jinbao Wang, Xiuqing Zhang, Hui Jiang, Fengping Xu, Lijin Zou, Huajing Teng, Xin Liu, Xun Xu, Jian Wang, Huanming Yang, Lars Bolund, George M. Church, Lin Lin, Yonglun Luo
doi: https://doi.org/10.1101/2020.05.20.103614
Xi Xiang
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
2BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
3BGI-Shenzhen, Shenzhen 518083, China
4Department of Biomedicine, Aarhus University, Aarhus 8000, Denmark
Kunli Qu
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
5Qingdao-Europe Advanced Institute for Life Sciences, BGI-Shenzhen, Qingdao 266555, China
Xue Liang
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
5Qingdao-Europe Advanced Institute for Life Sciences, BGI-Shenzhen, Qingdao 266555, China
Xiaoguang Pan
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
5Qingdao-Europe Advanced Institute for Life Sciences, BGI-Shenzhen, Qingdao 266555, China
Jun Wang
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
2BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
3BGI-Shenzhen, Shenzhen 518083, China
Peng Han
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
2BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
5Qingdao-Europe Advanced Institute for Life Sciences, BGI-Shenzhen, Qingdao 266555, China
Zhanying Dong
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
Lijun Liu
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
5Qingdao-Europe Advanced Institute for Life Sciences, BGI-Shenzhen, Qingdao 266555, China
Jiayan Zhong
6MGI, BGI-Shenzhen, Shenzhen 518083, China
Tao Ma
6MGI, BGI-Shenzhen, Shenzhen 518083, China
Yiqing Wang
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
Jiaying Yu
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
2BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
Xiaoying Zhao
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
2BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
Siyuan Li
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
2BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
Zhe Xu
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
2BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
Jinbao Wang
6MGI, BGI-Shenzhen, Shenzhen 518083, China
Xiuqing Zhang
2BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
3BGI-Shenzhen, Shenzhen 518083, China
Hui Jiang
6MGI, BGI-Shenzhen, Shenzhen 518083, China
Fengping Xu
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
3BGI-Shenzhen, Shenzhen 518083, China
Lijin Zou
7The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
Huajing Teng
8Key Laboratory of Carcinogenesis and Translational Research, Department of Radiation Oncology, Peking University Cancer Hospital & Institute, Beijing, China
Xin Liu
3BGI-Shenzhen, Shenzhen 518083, China
Xun Xu
3BGI-Shenzhen, Shenzhen 518083, China
9Guangdong Provincial Key Laboratory of Genome Read and Write,BGI-Shenzhen, Shenzhen, 518120,China
Jian Wang
3BGI-Shenzhen, Shenzhen 518083, China
Huanming Yang
3BGI-Shenzhen, Shenzhen 518083, China
10Guangdong Provincial Academician Workstation of BGI Synthetic Genomics,BGI-Shenzhen, Shenzhen, 518120,China
Lars Bolund
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
3BGI-Shenzhen, Shenzhen 518083, China
4Department of Biomedicine, Aarhus University, Aarhus 8000, Denmark
5Qingdao-Europe Advanced Institute for Life Sciences, BGI-Shenzhen, Qingdao 266555, China
George M. Church
11Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA
Lin Lin
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
4Department of Biomedicine, Aarhus University, Aarhus 8000, Denmark
12Steno Diabetes Center Aarhus, Aarhus University, Aarhus 8200, Denmark
Yonglun Luo
1Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China
3BGI-Shenzhen, Shenzhen 518083, China
4Department of Biomedicine, Aarhus University, Aarhus 8000, Denmark
5Qingdao-Europe Advanced Institute for Life Sciences, BGI-Shenzhen, Qingdao 266555, China
12Steno Diabetes Center Aarhus, Aarhus University, Aarhus 8200, Denmark
Article usage
Posted May 21, 2020.
Massively parallel quantification of CRISPR editing in cells by TRAP-seq enables better design of Cas9, ABE, CBE gRNAs of high efficiency and accuracy
Xi Xiang, Kunli Qu, Xue Liang, Xiaoguang Pan, Jun Wang, Peng Han, Zhanying Dong, Lijun Liu, Jiayan Zhong, Tao Ma, Yiqing Wang, Jiaying Yu, Xiaoying Zhao, Siyuan Li, Zhe Xu, Jinbao Wang, Xiuqing Zhang, Hui Jiang, Fengping Xu, Lijin Zou, Huajing Teng, Xin Liu, Xun Xu, Jian Wang, Huanming Yang, Lars Bolund, George M. Church, Lin Lin, Yonglun Luo
bioRxiv 2020.05.20.103614; doi: https://doi.org/10.1101/2020.05.20.103614
Massively parallel quantification of CRISPR editing in cells by TRAP-seq enables better design of Cas9, ABE, CBE gRNAs of high efficiency and accuracy
Xi Xiang, Kunli Qu, Xue Liang, Xiaoguang Pan, Jun Wang, Peng Han, Zhanying Dong, Lijun Liu, Jiayan Zhong, Tao Ma, Yiqing Wang, Jiaying Yu, Xiaoying Zhao, Siyuan Li, Zhe Xu, Jinbao Wang, Xiuqing Zhang, Hui Jiang, Fengping Xu, Lijin Zou, Huajing Teng, Xin Liu, Xun Xu, Jian Wang, Huanming Yang, Lars Bolund, George M. Church, Lin Lin, Yonglun Luo
bioRxiv 2020.05.20.103614; doi: https://doi.org/10.1101/2020.05.20.103614
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