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Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy

Hammad A. Faizi, Cody J. Reeves, Vasil N. Georgiev, View ORCID ProfilePetia M. Vlahovska, View ORCID ProfileRumiana Dimova
doi: https://doi.org/10.1101/2020.05.20.104240
Hammad A. Faizi
aDepartment of Mechanical Engineering, Northwestern University, Evanston, IL 60208, USA
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Cody J. Reeves
cDepartment of Engineering Sciences and Applied Mathematics, Northwestern University, Evanston, IL 60208, USA
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Vasil N. Georgiev
bDepartment of Theory and Biosystems, Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14424 Potsdam, Germany
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Petia M. Vlahovska
cDepartment of Engineering Sciences and Applied Mathematics, Northwestern University, Evanston, IL 60208, USA
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  • ORCID record for Petia M. Vlahovska
  • For correspondence: Rumiana.Dimova@mpikg.mpg.de Petia.Vlahovska@northwestern.edu
Rumiana Dimova
bDepartment of Theory and Biosystems, Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14424 Potsdam, Germany
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  • ORCID record for Rumiana Dimova
  • For correspondence: Rumiana.Dimova@mpikg.mpg.de Petia.Vlahovska@northwestern.edu
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Abstract

A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted May 22, 2020.
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Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy
Hammad A. Faizi, Cody J. Reeves, Vasil N. Georgiev, Petia M. Vlahovska, Rumiana Dimova
bioRxiv 2020.05.20.104240; doi: https://doi.org/10.1101/2020.05.20.104240
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Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy
Hammad A. Faizi, Cody J. Reeves, Vasil N. Georgiev, Petia M. Vlahovska, Rumiana Dimova
bioRxiv 2020.05.20.104240; doi: https://doi.org/10.1101/2020.05.20.104240

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