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A natural single-guide RNA repurposes Cas9 to autoregulate CRISPR-Cas expression

Rachael E. Workman, Teja Pammi, Binh T. K. Nguyen, Leonardo W. Graeff, Erika Smith, Suzanne M. Sebald, Marie J. Stoltzfus, View ORCID ProfileJoshua W. Modell
doi: https://doi.org/10.1101/2020.05.21.102756
Rachael E. Workman
1Department of Molecular Biology & Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
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Teja Pammi
1Department of Molecular Biology & Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
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Binh T. K. Nguyen
1Department of Molecular Biology & Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
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Leonardo W. Graeff
1Department of Molecular Biology & Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
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Erika Smith
2Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
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Suzanne M. Sebald
1Department of Molecular Biology & Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
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Marie J. Stoltzfus
1Department of Molecular Biology & Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
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Joshua W. Modell
1Department of Molecular Biology & Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
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  • ORCID record for Joshua W. Modell
  • For correspondence: jmodell1@jhmi.edu
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Posted May 21, 2020.
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A natural single-guide RNA repurposes Cas9 to autoregulate CRISPR-Cas expression
Rachael E. Workman, Teja Pammi, Binh T. K. Nguyen, Leonardo W. Graeff, Erika Smith, Suzanne M. Sebald, Marie J. Stoltzfus, Joshua W. Modell
bioRxiv 2020.05.21.102756; doi: https://doi.org/10.1101/2020.05.21.102756
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A natural single-guide RNA repurposes Cas9 to autoregulate CRISPR-Cas expression
Rachael E. Workman, Teja Pammi, Binh T. K. Nguyen, Leonardo W. Graeff, Erika Smith, Suzanne M. Sebald, Marie J. Stoltzfus, Joshua W. Modell
bioRxiv 2020.05.21.102756; doi: https://doi.org/10.1101/2020.05.21.102756

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