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Cell-state transitions and collective cell movement generate an endoderm-like region in gastruloids

Ali Hashmi, Sham Tlili, Pierre Perrin, Alfonso Martinez-Arias, View ORCID ProfilePierre-François Lenne
doi: https://doi.org/10.1101/2020.05.21.105551
Ali Hashmi
1Aix-Marseille University, CNRS, UMR 7288, IBDM, Turing Center for Living Systems, Marseille, France
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Sham Tlili
1Aix-Marseille University, CNRS, UMR 7288, IBDM, Turing Center for Living Systems, Marseille, France
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Pierre Perrin
1Aix-Marseille University, CNRS, UMR 7288, IBDM, Turing Center for Living Systems, Marseille, France
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Alfonso Martinez-Arias
2Department of Genetics, University of Cambridge, United Kingdom
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Pierre-François Lenne
1Aix-Marseille University, CNRS, UMR 7288, IBDM, Turing Center for Living Systems, Marseille, France
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  • ORCID record for Pierre-François Lenne
  • For correspondence: pierre-francois.lenne@univ-amu.fr
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Abstract

Shaping the animal body plan is a complex process that involves the spatial organization and patterning of different layers of cells. Recent advances in live imaging have started to unravel the cellular choreography underlying this process in mammals, however, the sequence of events transforming an unpatterned group of cells into structured territories is largely unknown. Here, using 3D aggregates of mouse embryonic stem cells, we study the formation of one of the three germ layers, the endoderm. We show that the endoderm can be generated from an epiblast-like state by a three-tier mechanism: a release of islands of E-cadherin expressing cells, their flow toward the tip of the 3D aggregate, and their segregation. In contrast to the prevailing view, this mechanism does not require epithelial to mesenchymal transitions and vice-versa but rather a fragmentation, which is mediated by Wnt/β- catenin, and a sorting process. Our data emphasize the role of signaling and cell flows in the establishment of the body plan.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 24, 2020.
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Cell-state transitions and collective cell movement generate an endoderm-like region in gastruloids
Ali Hashmi, Sham Tlili, Pierre Perrin, Alfonso Martinez-Arias, Pierre-François Lenne
bioRxiv 2020.05.21.105551; doi: https://doi.org/10.1101/2020.05.21.105551
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Cell-state transitions and collective cell movement generate an endoderm-like region in gastruloids
Ali Hashmi, Sham Tlili, Pierre Perrin, Alfonso Martinez-Arias, Pierre-François Lenne
bioRxiv 2020.05.21.105551; doi: https://doi.org/10.1101/2020.05.21.105551

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