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A kinetic model improves off-target predictions and reveals the physical basis of SpCas9 fidelity
Behrouz Eslami-Mossallam, Misha Klein, Constantijn v.d. Smagt, Koen v.d. Sanden, Stephen K. Jones Jr., John A. Hawkins, View ORCID ProfileIlya J. Finkelstein, Martin Depken
doi: https://doi.org/10.1101/2020.05.21.108613
Behrouz Eslami-Mossallam
1Kavli Institute of NanoScience and Department of BioNanoScience, Delft University of Technology, Delft 2629HZ, the Netherlands
Misha Klein
1Kavli Institute of NanoScience and Department of BioNanoScience, Delft University of Technology, Delft 2629HZ, the Netherlands
Constantijn v.d. Smagt
1Kavli Institute of NanoScience and Department of BioNanoScience, Delft University of Technology, Delft 2629HZ, the Netherlands
Koen v.d. Sanden
1Kavli Institute of NanoScience and Department of BioNanoScience, Delft University of Technology, Delft 2629HZ, the Netherlands
Stephen K. Jones Jr.
2Department of Molecular Biosciences, University of Texas at Austin, Austin, Texas 78712, USA
3Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA
4Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas 78712, USA
John A. Hawkins
2Department of Molecular Biosciences, University of Texas at Austin, Austin, Texas 78712, USA
3Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA
4Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas 78712, USA
5Oden Institute for Computational Engineering and Science, University of Texas at Austin, Austin, Texas 78712, USA
Ilya J. Finkelstein
2Department of Molecular Biosciences, University of Texas at Austin, Austin, Texas 78712, USA
3Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA
4Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas 78712, USA
Martin Depken
1Kavli Institute of NanoScience and Department of BioNanoScience, Delft University of Technology, Delft 2629HZ, the Netherlands
Posted July 21, 2020.
A kinetic model improves off-target predictions and reveals the physical basis of SpCas9 fidelity
Behrouz Eslami-Mossallam, Misha Klein, Constantijn v.d. Smagt, Koen v.d. Sanden, Stephen K. Jones Jr., John A. Hawkins, Ilya J. Finkelstein, Martin Depken
bioRxiv 2020.05.21.108613; doi: https://doi.org/10.1101/2020.05.21.108613
A kinetic model improves off-target predictions and reveals the physical basis of SpCas9 fidelity
Behrouz Eslami-Mossallam, Misha Klein, Constantijn v.d. Smagt, Koen v.d. Sanden, Stephen K. Jones Jr., John A. Hawkins, Ilya J. Finkelstein, Martin Depken
bioRxiv 2020.05.21.108613; doi: https://doi.org/10.1101/2020.05.21.108613
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