Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Subcellular resolution 3D light field imaging with genetically encoded voltage indicators

View ORCID ProfilePeter Quicke, View ORCID ProfileCarmel L. Howe, Pingfan Song, Herman Verinaz Jadan, Chenchen Song, View ORCID ProfileThomas Knöpfel, View ORCID ProfileMark Neil, View ORCID ProfilePier Luigi Dragotti, View ORCID ProfileSimon R. Schultz, View ORCID ProfileAmanda J. Foust
doi: https://doi.org/10.1101/2020.05.22.108191
Peter Quicke
aDepartment of Bioengineering, Imperial College London, London, UK
bCentre for Neurotechnology, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Peter Quicke
Carmel L. Howe
aDepartment of Bioengineering, Imperial College London, London, UK
bCentre for Neurotechnology, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Carmel L. Howe
Pingfan Song
cDepartment of Electrical and Electronic Engineering, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Herman Verinaz Jadan
cDepartment of Electrical and Electronic Engineering, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chenchen Song
dDepartment of Brain Sciences, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thomas Knöpfel
dDepartment of Brain Sciences, Imperial College London, London, UK
bCentre for Neurotechnology, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Thomas Knöpfel
Mark Neil
eDepartment of Physics, Imperial College London, London, UK
bCentre for Neurotechnology, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mark Neil
Pier Luigi Dragotti
cDepartment of Electrical and Electronic Engineering, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Pier Luigi Dragotti
Simon R. Schultz
aDepartment of Bioengineering, Imperial College London, London, UK
bCentre for Neurotechnology, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Simon R. Schultz
Amanda J. Foust
aDepartment of Bioengineering, Imperial College London, London, UK
bCentre for Neurotechnology, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Amanda J. Foust
  • For correspondence: a.foust@ic.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Light field microscopy (LFM) enables high signal-to-noise ratio (SNR), light efficient volume imaging at fast frame rates, and has been successfully applied to single-cell resolution functional neuronal calcium imaging. Voltage imaging with genetically encoded voltage indicators (GEVIs) stands to particularly benefit from light field microscopy’s volumetric imaging capability due to high required sampling rates, and limited probe brightness and functional sensitivity. Previous LFM studies have imaged GEVIs to track population-level interactions only in invertebrate preparations and without single cell resolution. Here we demonstrate sub-cellular resolution GEVI light field imaging in acute mouse brain slices resolving dendritic voltage signals localized in three dimensions. We characterize the effects of different light field reconstruction techniques on the SNR and signal localization and compare the SNR to fluorescence transients imaged in wide field. Our results demonstrate the potential of light field voltage imaging for studying dendritic integration and action potential propagation and backpropagation in 3 spatial dimensions.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted May 25, 2020.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Subcellular resolution 3D light field imaging with genetically encoded voltage indicators
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Subcellular resolution 3D light field imaging with genetically encoded voltage indicators
Peter Quicke, Carmel L. Howe, Pingfan Song, Herman Verinaz Jadan, Chenchen Song, Thomas Knöpfel, Mark Neil, Pier Luigi Dragotti, Simon R. Schultz, Amanda J. Foust
bioRxiv 2020.05.22.108191; doi: https://doi.org/10.1101/2020.05.22.108191
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Subcellular resolution 3D light field imaging with genetically encoded voltage indicators
Peter Quicke, Carmel L. Howe, Pingfan Song, Herman Verinaz Jadan, Chenchen Song, Thomas Knöpfel, Mark Neil, Pier Luigi Dragotti, Simon R. Schultz, Amanda J. Foust
bioRxiv 2020.05.22.108191; doi: https://doi.org/10.1101/2020.05.22.108191

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (3701)
  • Biochemistry (7820)
  • Bioengineering (5695)
  • Bioinformatics (21343)
  • Biophysics (10603)
  • Cancer Biology (8206)
  • Cell Biology (11974)
  • Clinical Trials (138)
  • Developmental Biology (6786)
  • Ecology (10425)
  • Epidemiology (2065)
  • Evolutionary Biology (13908)
  • Genetics (9731)
  • Genomics (13109)
  • Immunology (8171)
  • Microbiology (20064)
  • Molecular Biology (7875)
  • Neuroscience (43171)
  • Paleontology (321)
  • Pathology (1282)
  • Pharmacology and Toxicology (2267)
  • Physiology (3363)
  • Plant Biology (7254)
  • Scientific Communication and Education (1316)
  • Synthetic Biology (2012)
  • Systems Biology (5550)
  • Zoology (1133)