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Features Of Hippocampal Astrocytic Domains And Their Spatial Relation To Excitatory And Inhibitory Neurons

Ron Refaeli, Adi Doron, Aviya Benmelech-Chovav, Maya Groysman, Tirzah Kreisel, View ORCID ProfileYonatan Loewenstein, View ORCID ProfileInbal Goshen
doi: https://doi.org/10.1101/2020.05.25.114348
Ron Refaeli
1Edmond and Lily Safra Center for Brain Sciences (ELSC), The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
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Adi Doron
1Edmond and Lily Safra Center for Brain Sciences (ELSC), The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
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Aviya Benmelech-Chovav
1Edmond and Lily Safra Center for Brain Sciences (ELSC), The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
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Maya Groysman
2ELSC Vector Core Facility, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
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Tirzah Kreisel
1Edmond and Lily Safra Center for Brain Sciences (ELSC), The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
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Yonatan Loewenstein
1Edmond and Lily Safra Center for Brain Sciences (ELSC), The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
3Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
4Federmann Center for the Study of Rationality, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
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Inbal Goshen
1Edmond and Lily Safra Center for Brain Sciences (ELSC), The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
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  • ORCID record for Inbal Goshen
  • For correspondence: inbal.goshen@elsc.huji.ac.il
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SUMMARY

The mounting evidence for the involvement of astrocytes in neuronal circuits function and behavior stands in stark contrast to the lack of detailed anatomical description of these cells and the neurons in their domains. To fill this void, we imaged >30,000 astrocytes in cleared hippocampi, and employed converging genetic, histological and computational tools to determine the elaborate structure, distribution and neuronal content of astrocytic domains. First, we characterized the spatial distribution of >19,000 astrocytes across CA1 lamina, and analyzed the detailed morphology of thousands of reconstructed domains. We then determined the excitatory content of CA1 astrocytes, averaging above 13 pyramidal neurons per domain and increasing towards CA1 midline. Finally, we discovered that somatostatin neurons are found in close proximity to astrocytes, compared to parvalbumin and VIP inhibitory neurons. This resource expands our understanding of fundamental hippocampal design principles, and provides the first quantitative foundation for neuron-astrocyte interactions in this region.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted May 25, 2020.
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Features Of Hippocampal Astrocytic Domains And Their Spatial Relation To Excitatory And Inhibitory Neurons
Ron Refaeli, Adi Doron, Aviya Benmelech-Chovav, Maya Groysman, Tirzah Kreisel, Yonatan Loewenstein, Inbal Goshen
bioRxiv 2020.05.25.114348; doi: https://doi.org/10.1101/2020.05.25.114348
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Features Of Hippocampal Astrocytic Domains And Their Spatial Relation To Excitatory And Inhibitory Neurons
Ron Refaeli, Adi Doron, Aviya Benmelech-Chovav, Maya Groysman, Tirzah Kreisel, Yonatan Loewenstein, Inbal Goshen
bioRxiv 2020.05.25.114348; doi: https://doi.org/10.1101/2020.05.25.114348

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