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Novel heme-binding enables allosteric modulation in an ancient TIM-barrel glycosidase

Gloria Gamiz-Arco, Luis I. Gutierrez-Rus, Valeria A. Risso, Beatriz Ibarra-Molero, Yosuke Hoshino, Dušan Petrović, Adrian Romero-Rivera, Burckhard Seelig, Jose A. Gavira, Shina C.L. Kamerlin, Eric A. Gaucher, Jose M. Sanchez-Ruiz
doi: https://doi.org/10.1101/2020.05.27.118968
Gloria Gamiz-Arco
1Departamento de Quimica Fisica. Facultad de Ciencias, Unidad de Excelencia de Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, 18071 Granada, Spain
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Luis I. Gutierrez-Rus
1Departamento de Quimica Fisica. Facultad de Ciencias, Unidad de Excelencia de Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, 18071 Granada, Spain
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Valeria A. Risso
1Departamento de Quimica Fisica. Facultad de Ciencias, Unidad de Excelencia de Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, 18071 Granada, Spain
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Beatriz Ibarra-Molero
1Departamento de Quimica Fisica. Facultad de Ciencias, Unidad de Excelencia de Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, 18071 Granada, Spain
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Yosuke Hoshino
2Department of Biology, Georgia State University, Atlanta, GA 30306 U.S.A.
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Dušan Petrović
3Science for Life Laboratory, Department of Chemistry-BMC, Uppsala University, BMC Box 576, S-751 23 Uppsala, Sweden
7Hit Discovery, Discovery Sciences, Biopharmaceutical R&D, AstraZeneca, 431 50 Gothenburg, Sweden
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Adrian Romero-Rivera
3Science for Life Laboratory, Department of Chemistry-BMC, Uppsala University, BMC Box 576, S-751 23 Uppsala, Sweden
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Burckhard Seelig
4Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, United States of America, & BioTechnology Institute, University of Minnesota, St. Paul, Minnesota, United States of America
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Jose A. Gavira
5Laboratorio de Estudios Cristalograficos, Instituto Andaluz de Ciencias de la Tierra, CSIC, Unidad de Excelencia de Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, Avenida de las Palmeras 4, Granada 18100 Armilla, Spain
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Shina C.L. Kamerlin
3Science for Life Laboratory, Department of Chemistry-BMC, Uppsala University, BMC Box 576, S-751 23 Uppsala, Sweden
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Eric A. Gaucher
2Department of Biology, Georgia State University, Atlanta, GA 30306 U.S.A.
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Jose M. Sanchez-Ruiz
1Departamento de Quimica Fisica. Facultad de Ciencias, Unidad de Excelencia de Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, 18071 Granada, Spain
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  • For correspondence: sanchezr@ugr.es
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Article Information

doi 
https://doi.org/10.1101/2020.05.27.118968
History 
  • May 28, 2020.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.

Author Information

  1. Gloria Gamiz-Arco1,6,
  2. Luis I. Gutierrez-Rus1,6,
  3. Valeria A. Risso1,
  4. Beatriz Ibarra-Molero1,
  5. Yosuke Hoshino2,
  6. Dušan Petrović3,7,
  7. Adrian Romero-Rivera3,
  8. Burckhard Seelig4,
  9. Jose A. Gavira5,
  10. Shina C.L. Kamerlin3,*,
  11. Eric A. Gaucher2,* and
  12. Jose M. Sanchez-Ruiz1,*,#
  1. 1Departamento de Quimica Fisica. Facultad de Ciencias, Unidad de Excelencia de Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, 18071 Granada, Spain
  2. 2Department of Biology, Georgia State University, Atlanta, GA 30306 U.S.A.
  3. 3Science for Life Laboratory, Department of Chemistry-BMC, Uppsala University, BMC Box 576, S-751 23 Uppsala, Sweden
  4. 4Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, United States of America, & BioTechnology Institute, University of Minnesota, St. Paul, Minnesota, United States of America
  5. 5Laboratorio de Estudios Cristalograficos, Instituto Andaluz de Ciencias de la Tierra, CSIC, Unidad de Excelencia de Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, Avenida de las Palmeras 4, Granada 18100 Armilla, Spain
  6. 7Hit Discovery, Discovery Sciences, Biopharmaceutical R&D, AstraZeneca, 431 50 Gothenburg, Sweden
  1. ↵#Corresponding author; email: sanchezr{at}ugr.es
  1. ↵6 These authors contributed equally to this work

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Novel heme-binding enables allosteric modulation in an ancient TIM-barrel glycosidase
Gloria Gamiz-Arco, Luis I. Gutierrez-Rus, Valeria A. Risso, Beatriz Ibarra-Molero, Yosuke Hoshino, Dušan Petrović, Adrian Romero-Rivera, Burckhard Seelig, Jose A. Gavira, Shina C.L. Kamerlin, Eric A. Gaucher, Jose M. Sanchez-Ruiz
bioRxiv 2020.05.27.118968; doi: https://doi.org/10.1101/2020.05.27.118968
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Novel heme-binding enables allosteric modulation in an ancient TIM-barrel glycosidase
Gloria Gamiz-Arco, Luis I. Gutierrez-Rus, Valeria A. Risso, Beatriz Ibarra-Molero, Yosuke Hoshino, Dušan Petrović, Adrian Romero-Rivera, Burckhard Seelig, Jose A. Gavira, Shina C.L. Kamerlin, Eric A. Gaucher, Jose M. Sanchez-Ruiz
bioRxiv 2020.05.27.118968; doi: https://doi.org/10.1101/2020.05.27.118968

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