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Remnants of the Balbiani body are required for formation of RNA transport granules in Xenopus oocytes

Chao Yang, Gena M. Wilson, View ORCID ProfileMatthew M. Champion, View ORCID ProfilePaul W. Huber
doi: https://doi.org/10.1101/2020.05.27.119487
Chao Yang
1Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA
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Gena M. Wilson
1Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA
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Matthew M. Champion
1Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA
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Paul W. Huber
1Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA
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  • For correspondence: phuber@nd.edu
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Abstract

Fragmentation of the Balbiani body in Xenopus oocytes engenders a region extending from the germinal vesicle (GV) towards the vegetal pole that is enriched in mitochondria. This area is later transversed by RNA that is being localized to the vegetal cortex. Inhibition of mitochondrial ATP synthesis prevents the perinuclear formation of these RNA transport granules that can be reversed by the nonhydrolyzable ATP analog, adenosine 5’-(βγ-imido) triphosphate. The protein composition and sensitivity of the transport granules to hexanediol indicate that they are liquid phase condensates. Mitochondria in the remnants of the Balbiani body produce a region of elevated ATP that appears to act as a hydrotrope to support the perinuclear phase transition leading to granule formation.

Summary Mitochondria in the remnants of the Balbiani body produce elevated levels of ATP required for the formation of liquid phase condensates containing RNA transport granules.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted May 30, 2020.
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Remnants of the Balbiani body are required for formation of RNA transport granules in Xenopus oocytes
Chao Yang, Gena M. Wilson, Matthew M. Champion, Paul W. Huber
bioRxiv 2020.05.27.119487; doi: https://doi.org/10.1101/2020.05.27.119487
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Remnants of the Balbiani body are required for formation of RNA transport granules in Xenopus oocytes
Chao Yang, Gena M. Wilson, Matthew M. Champion, Paul W. Huber
bioRxiv 2020.05.27.119487; doi: https://doi.org/10.1101/2020.05.27.119487

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