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An image resource of subdivided Drosophila GAL4-driver expression patterns for neuron-level searches

View ORCID ProfileGeoffrey W. Meissner, Zachary Dorman, View ORCID ProfileAljoscha Nern, Kaitlyn Forster, View ORCID ProfileTheresa Gibney, Jennifer Jeter, Lauren Johnson, Yisheng He, Kelley Lee, Brian Melton, Brianna Yarbrough, View ORCID ProfileJody Clements, View ORCID ProfileCristian Goina, View ORCID ProfileHideo Otsuna, View ORCID ProfileKonrad Rokicki, View ORCID ProfileRobert R. Svirskas, Yoshinori Aso, Gwyneth M. Card, View ORCID ProfileBarry J. Dickson, View ORCID ProfileErica Ehrhardt, View ORCID ProfileJens Goldammer, Masayoshi Ito, View ORCID ProfileWyatt Korff, View ORCID ProfileRyo Minegishi, View ORCID ProfileShigehiro Namiki, View ORCID ProfileGerald M. Rubin, View ORCID ProfileGabriella Sterne, Tanya Wolff, Oz Malkesman, FlyLight Project Team
doi: https://doi.org/10.1101/2020.05.29.080473
Geoffrey W. Meissner
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, United States
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  • For correspondence: meissnerg@janelia.hhmi.org asoy@janelia.hhmi.org cardg@janelia.hhmi.org dicksonb@janelia.hhmi.org korffw@janelia.hhmi.org rubing@janelia.hhmi.org
Zachary Dorman
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Aljoscha Nern
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Kaitlyn Forster
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Theresa Gibney
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Jennifer Jeter
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Lauren Johnson
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Yisheng He
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Kelley Lee
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Brian Melton
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Brianna Yarbrough
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Jody Clements
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Cristian Goina
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Hideo Otsuna
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Konrad Rokicki
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Robert R. Svirskas
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Yoshinori Aso
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  • For correspondence: meissnerg@janelia.hhmi.org asoy@janelia.hhmi.org cardg@janelia.hhmi.org dicksonb@janelia.hhmi.org korffw@janelia.hhmi.org rubing@janelia.hhmi.org
Gwyneth M. Card
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Barry J. Dickson
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  • For correspondence: meissnerg@janelia.hhmi.org asoy@janelia.hhmi.org cardg@janelia.hhmi.org dicksonb@janelia.hhmi.org korffw@janelia.hhmi.org rubing@janelia.hhmi.org
Erica Ehrhardt
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Jens Goldammer
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Masayoshi Ito
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Wyatt Korff
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  • For correspondence: meissnerg@janelia.hhmi.org asoy@janelia.hhmi.org cardg@janelia.hhmi.org dicksonb@janelia.hhmi.org korffw@janelia.hhmi.org rubing@janelia.hhmi.org
Ryo Minegishi
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Shigehiro Namiki
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Gerald M. Rubin
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  • For correspondence: meissnerg@janelia.hhmi.org asoy@janelia.hhmi.org cardg@janelia.hhmi.org dicksonb@janelia.hhmi.org korffw@janelia.hhmi.org rubing@janelia.hhmi.org
Gabriella Sterne
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Tanya Wolff
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Oz Malkesman
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Abstract

Precise, repeatable genetic access to specific neurons via the GAL4/UAS system and related methods is a key advantage of Drosophila neuroscience. Neuronal targeting is typically documented using light microscopy of full GAL4 expression patterns, which mostly lack the single-cell resolution required for reliable cell type identification. Here we use stochastic GAL4 labeling with the MultiColor FlpOut approach to generate cellular resolution confocal images at large scale. We are releasing aligned images of 27,000 such adult central nervous systems.

An anticipated use of this resource is to bridge the gap between electron microscopy-identified neurons and light microscopy-based intersectional genetic approaches such as the split-GAL4 system. Identifying the individual neurons that make up each GAL4 expression pattern improves the prediction of which GAL4 enhancer fragments best combine via split-GAL4 to target neurons of interest. To this end we have developed the NeuronBridge search tool, which matches these light microscope neuronal images to neurons in the recently published FlyEM hemibrain. This work thus provides a resource and search tool that will significantly enhance both the efficiency and efficacy of split-GAL4 targeting of EM-identified neurons and further advance Drosophila neuroscience.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://data.janelia.org/ctEpc

  • https://data.janelia.org/sk7zb

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted May 30, 2020.
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An image resource of subdivided Drosophila GAL4-driver expression patterns for neuron-level searches
Geoffrey W. Meissner, Zachary Dorman, Aljoscha Nern, Kaitlyn Forster, Theresa Gibney, Jennifer Jeter, Lauren Johnson, Yisheng He, Kelley Lee, Brian Melton, Brianna Yarbrough, Jody Clements, Cristian Goina, Hideo Otsuna, Konrad Rokicki, Robert R. Svirskas, Yoshinori Aso, Gwyneth M. Card, Barry J. Dickson, Erica Ehrhardt, Jens Goldammer, Masayoshi Ito, Wyatt Korff, Ryo Minegishi, Shigehiro Namiki, Gerald M. Rubin, Gabriella Sterne, Tanya Wolff, Oz Malkesman, FlyLight Project Team
bioRxiv 2020.05.29.080473; doi: https://doi.org/10.1101/2020.05.29.080473
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An image resource of subdivided Drosophila GAL4-driver expression patterns for neuron-level searches
Geoffrey W. Meissner, Zachary Dorman, Aljoscha Nern, Kaitlyn Forster, Theresa Gibney, Jennifer Jeter, Lauren Johnson, Yisheng He, Kelley Lee, Brian Melton, Brianna Yarbrough, Jody Clements, Cristian Goina, Hideo Otsuna, Konrad Rokicki, Robert R. Svirskas, Yoshinori Aso, Gwyneth M. Card, Barry J. Dickson, Erica Ehrhardt, Jens Goldammer, Masayoshi Ito, Wyatt Korff, Ryo Minegishi, Shigehiro Namiki, Gerald M. Rubin, Gabriella Sterne, Tanya Wolff, Oz Malkesman, FlyLight Project Team
bioRxiv 2020.05.29.080473; doi: https://doi.org/10.1101/2020.05.29.080473

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