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A chromosome-level genome assembly of the woolly apple aphid, Eriosoma lanigerum (Hausmann) (Hemiptera: Aphididae)

View ORCID ProfileRoberto Biello, View ORCID ProfileArchana Singh, Cindayniah J. Godfrey, Felicidad Fernández Fernández, View ORCID ProfileSam T. Mugford, Glen Powell, View ORCID ProfileSaskia A. Hogenhout, View ORCID ProfileThomas C. Mathers
doi: https://doi.org/10.1101/2020.05.29.121947
Roberto Biello
1Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, United Kingdom
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Archana Singh
2Earlham Institute, John Innes Centre, Norwich Research Park, Norwich, United Kingdom
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Cindayniah J. Godfrey
3NIAB EMR, New Road, East Malling, Kent, ME19 6BJ, United Kingdom
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Felicidad Fernández Fernández
3NIAB EMR, New Road, East Malling, Kent, ME19 6BJ, United Kingdom
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Sam T. Mugford
1Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, United Kingdom
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Glen Powell
3NIAB EMR, New Road, East Malling, Kent, ME19 6BJ, United Kingdom
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Saskia A. Hogenhout
1Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, United Kingdom
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Thomas C. Mathers
1Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, United Kingdom
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  • ORCID record for Thomas C. Mathers
  • For correspondence: thomas.mathers@jic.ac.uk
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ABSTRACT

Woolly apple aphid (WAA, Eriosoma lanigerum Hausmann) (Hemiptera: Aphididae), is a major pest of apple trees (Malus domestica, order Rosales) and is critical to the economics of the apple industry in most parts of the world. Here, we generated a chromosome-level genome assembly of WAA – representing the first genome sequence from the aphid subfamily Eriosomatinae – using a combination of 10X Genomics linked-reads and in vivo Hi-C data. The final genome assembly is 327 Mb, with 91% of the assembled sequences anchored into six chromosomes. The contig and scaffold N50 values are 158 kb and 71 Mb, respectively, and we predicted a total of 28,186 protein-coding genes. The assembly is highly complete, including 97% of conserved arthropod single-copy orthologues based on BUSCO analysis. Phylogenomic analysis of WAA and nine previously published aphid genomes, spanning four aphid tribes and three subfamilies, reveals that the tribe Eriosomatini (represented by WAA) is recovered as a sister group to Aphidini + Macrosiphini (subfamily Aphidinae). We identified syntenic blocks of genes between our WAA assembly and the genomes of other aphid species and find that two WAA chromosomes (El5 and El6) map to the conserved Macrosiphini and Aphidini X chromosome. Our high-quality WAA genome assembly and annotation provides a valuable resource for research in a broad range of areas such as comparative and population genomics, insect-plant interactions and pest resistance management.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://10.5281/zenodo.3797131

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 30, 2020.
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A chromosome-level genome assembly of the woolly apple aphid, Eriosoma lanigerum (Hausmann) (Hemiptera: Aphididae)
Roberto Biello, Archana Singh, Cindayniah J. Godfrey, Felicidad Fernández Fernández, Sam T. Mugford, Glen Powell, Saskia A. Hogenhout, Thomas C. Mathers
bioRxiv 2020.05.29.121947; doi: https://doi.org/10.1101/2020.05.29.121947
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A chromosome-level genome assembly of the woolly apple aphid, Eriosoma lanigerum (Hausmann) (Hemiptera: Aphididae)
Roberto Biello, Archana Singh, Cindayniah J. Godfrey, Felicidad Fernández Fernández, Sam T. Mugford, Glen Powell, Saskia A. Hogenhout, Thomas C. Mathers
bioRxiv 2020.05.29.121947; doi: https://doi.org/10.1101/2020.05.29.121947

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