ABSTRACT
Ewing sarcoma is driven by fusion proteins containing a low complexity (LC) domain that is intrinsically disordered and a powerful transcriptional regulator. The most common fusion protein found in Ewing sarcoma, EWS-FLI1, takes its LC domain from the RNA-binding protein EWSR1 (Ewing Sarcoma RNA-binding protein 1) and a DNA-binding domain from the transcription factor FLI1 (Friend Leukemia Virus Integration 1). EWS-FLI1 binds RNA polymerase II (RNA Pol II) and can self-assemble through a process known as phase separation. The ability of self-oligomerizing RNA-binding proteins like EWSR1 to assemble into ribonucleoprotein granules in cells has received significant attention but the role of phase separation in EWS-FLI1 activity is less understood. We investigated the intersecting roles of EWSR1 and EWS-FLI1 to control gene expression and tumorigenic cell growth in Ewing sarcoma. We also studied interactions among EWS-FLI1, EWSR1, and RNA Pol II. We applied a crosslinking approach to demonstrate the incorporation of EWSR1 and RNA Pol II into protein granules in cells. We also identified protein granules in cells associated with the fusion protein, EWS-FLI1. Interactions through the LC domain, which allow EWS-FLI1 to bind EWSR1 and RNA Pol II, were found to be required for inclusion into the cellular granules observed by TEM. The physical characterization of EWS-FLI1 assemblies reported here offers insight into a large protein assembly that may allow EWS-FLI1 to engage its wide network of protein partners while driving tumorigenesis.
Competing Interest Statement
The authors have declared no competing interest.