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Assignment of coronavirus spike protein site-specific glycosylation using GlycReSoft

View ORCID ProfileJoshua A. Klein, View ORCID ProfileJoseph Zaia
doi: https://doi.org/10.1101/2020.05.31.125302
Joshua A. Klein
1Bioinformatics Program, Boston University, Boston, MA USA
3Department of Biochemistry, Boston University, Boston, MA USA
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  • ORCID record for Joshua A. Klein
Joseph Zaia
1Bioinformatics Program, Boston University, Boston, MA USA
2Center for Biomedical Mass Spectrometry, Boston University, Boston, MA USA
3Department of Biochemistry, Boston University, Boston, MA USA
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  • For correspondence: jzaia@bu.edu
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Abstract

Widely-available LC-MS instruments and methods allow users to acquire glycoproteomics data. Complex glycans, however, add a dimension of complexity to the data analysis workflow. In a sense, complex glycans are post-translationally modified post-translational modifications, reflecting a series of biosynthetic reactions in the secretory pathway that are spatially and temporally regulated. One problem is that complex glycan is micro-heterogeneous, multiplying the complexity of the proteome. Another is that glycopeptide glycans undergo dissociation during tandem MS that must be considered for tandem MS interpretation algorithms and quantitative tools. Fortunately, there are a number of algorithmic tools available for analysis of glycoproteomics LC-MS data. We summarize the principles for glycopeptide data analysis and show use of our GlycReSoft tool to analyze SARS-CoV-2 spike protein site-specific glycosylation.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=061b094528a1427285e396d29c9dc4a4

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted May 31, 2020.
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Assignment of coronavirus spike protein site-specific glycosylation using GlycReSoft
Joshua A. Klein, Joseph Zaia
bioRxiv 2020.05.31.125302; doi: https://doi.org/10.1101/2020.05.31.125302
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Assignment of coronavirus spike protein site-specific glycosylation using GlycReSoft
Joshua A. Klein, Joseph Zaia
bioRxiv 2020.05.31.125302; doi: https://doi.org/10.1101/2020.05.31.125302

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